FastQCFastQC Report
Wed 18 Jan 2023
UA_Don_Mar_1_2021-08-23.trimmed1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUA_Don_Mar_1_2021-08-23.trimmed1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39985339
Sequences flagged as poor quality0
Sequence length75-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA3713240.9286503735781758No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT2909020.7275216548745529No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA2474560.6188668301649262No Hit
ACACAACACAACACAACACAACACAACACAACACAACACAACACAACACA2207290.5520248309011461No Hit
GTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTT1948500.48730360895527236No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACA1251080.31288468005735803No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTT1176130.29414030977704103No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT1082000.2705991813649498No Hit
CAACACAACACAACACAACACAACACAACACAACACAACACAACACAACA784900.19629694773876993No Hit
GAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATA764890.19129261352517232No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCT714130.17859796061751532No Hit
GTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGT685840.17152286741898073No Hit
ACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATA655920.1640401248067448No Hit
ATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTT633850.1585206017635614No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA446030.11154838527191177No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT434630.10869734029265077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)