FastQCFastQC Report
Wed 21 Dec 2022
DrosEu-143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDrosEu-143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39715499
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAACACAACACAACACAACACAACACAACACAACACAACACAACACA6461911.627049933327037No Hit
GTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTT5642511.4207324953917866No Hit
CAACACAACACAACACAACACAACACAACACAACACAACACAACACAACA2176640.5480580767724963No Hit
GTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGT1865950.46982917172965644No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTT1598210.4024146845039011No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACA1534410.38635042707130535No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1217270.3064974709243865No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA1054400.26548829211487435No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA622040.1566239920591203No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT548180.13802671848589892No Hit
GACAAAGACAAAGACAAAGACAAAGACAAAGACAAAGACAAAGACAAAGA435520.10965995920131837No Hit
GTCTTTGTCTTTGTCTTTGTCTTTGTCTTTGTCTTTGTCTTTGTCTTTGT412280.10380833940925682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)