## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/pipeline_output/FI_Pir_Aka_1_2019-08-25/FI_Pir_Aka_1_2019-08-25.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/pipeline_output/FI_Pir_Aka_1_2019-08-25/FI_Pir_Aka_1_2019-08-25.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/pipeline_output/FI_Pir_Aka_1_2019-08-25/FI_Pir_Aka_1_2019-08-25.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Wed Jan 18 04:07:30 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 2514700 23694038 0 0 1317008 7569757 204167 0.329772 29129908 ## HISTOGRAM java.lang.Double BIN VALUE all_sets optical_sets non_optical_sets 1.0 1.005633 10600640 0 10694035 2.0 1.451483 3429379 199207 3398505 3.0 1.649151 1060621 2181 1027010 4.0 1.736787 318119 48 300631 5.0 1.775641 93759 12 86499 6.0 1.792867 28719 2 26083 7.0 1.800504 9645 4 8723 8.0 1.80389 3610 1 3256 9.0 1.805391 1797 0 1689 10.0 1.806056 1060 1 983 11.0 1.806351 715 3 727 12.0 1.806482 571 2 538 13.0 1.80654 463 0 469 14.0 1.806566 393 2 375 15.0 1.806577 286 0 279 16.0 1.806582 274 0 273 17.0 1.806585 214 1 222 18.0 1.806586 178 1 166 19.0 1.806586 162 1 154 20.0 1.806586 130 0 134 21.0 1.806586 133 0 130 22.0 1.806586 113 0 111 23.0 1.806586 82 0 80 24.0 1.806586 64 0 64 25.0 1.806586 72 0 74 26.0 1.806586 81 0 82 27.0 1.806586 58 0 51 28.0 1.806586 62 0 61 29.0 1.806586 47 1 50 30.0 1.806586 37 0 35 31.0 1.806586 42 0 45 32.0 1.806586 31 0 23 33.0 1.806586 23 0 27 34.0 1.806586 31 0 32 35.0 1.806586 23 0 22 36.0 1.806586 24 0 24 37.0 1.806586 25 0 25 38.0 1.806586 23 0 22 39.0 1.806586 19 0 21 40.0 1.806586 19 0 17 41.0 1.806586 22 0 22 42.0 1.806586 18 0 19 43.0 1.806586 26 0 23 44.0 1.806586 17 0 16 45.0 1.806586 13 0 14 46.0 1.806586 11 0 11 47.0 1.806586 14 0 18 48.0 1.806586 17 0 18 49.0 1.806586 23 0 19 50.0 1.806586 14 0 13 51.0 1.806586 10 0 9 52.0 1.806586 8 0 12 53.0 1.806586 9 0 10 54.0 1.806586 18 0 13 55.0 1.806586 3 1 5 56.0 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