## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/pipeline_output/DK_Kar_1_Oct_2019/DK_Kar_1_Oct_2019.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/pipeline_output/DK_Kar_1_Oct_2019/DK_Kar_1_Oct_2019.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/pipeline_output/DK_Kar_1_Oct_2019/DK_Kar_1_Oct_2019.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Thu Dec 22 18:32:09 UTC 2022 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 3693516 24869268 0 0 2005331 6099302 258724 0.265832 43273978 ## HISTOGRAM java.lang.Double BIN VALUE all_sets optical_sets non_optical_sets 1.0 1.007782 13912210 0 14061422 2.0 1.57504 3441125 252653 3364354 3.0 1.894336 818886 2748 771970 4.0 2.074061 190545 51 172698 5.0 2.175224 44343 5 38909 6.0 2.232166 10605 9 8935 7.0 2.264218 2713 5 2339 8.0 2.282259 885 2 752 9.0 2.292414 400 1 367 10.0 2.29813 239 4 228 11.0 2.301347 189 2 177 12.0 2.303158 136 1 132 13.0 2.304178 131 1 128 14.0 2.304752 96 2 97 15.0 2.305075 72 2 75 16.0 2.305256 76 0 74 17.0 2.305359 57 2 57 18.0 2.305416 63 2 60 19.0 2.305449 40 0 40 20.0 2.305467 49 0 48 21.0 2.305477 32 0 33 22.0 2.305483 30 0 30 23.0 2.305486 27 0 26 24.0 2.305488 23 0 24 25.0 2.305489 32 0 31 26.0 2.30549 17 0 17 27.0 2.30549 25 0 24 28.0 2.30549 27 0 28 29.0 2.30549 18 0 18 30.0 2.30549 12 0 16 31.0 2.30549 20 0 16 32.0 2.30549 21 0 21 33.0 2.30549 18 0 18 34.0 2.30549 18 0 16 35.0 2.30549 13 1 14 36.0 2.30549 14 0 14 37.0 2.30549 13 0 14 38.0 2.30549 21 0 20 39.0 2.30549 7 0 8 40.0 2.30549 14 0 15 41.0 2.30549 10 0 10 42.0 2.30549 16 0 16 43.0 2.30549 10 0 9 44.0 2.30549 8 0 9 45.0 2.30549 9 0 9 46.0 2.30549 12 0 11 47.0 2.30549 8 0 7 48.0 2.30549 7 0 7 49.0 2.30549 10 0 12 50.0 2.30549 14 0 13 51.0 2.30549 9 0 8 52.0 2.30549 2 0 3 53.0 2.30549 7 0 6 54.0 2.30549 9 0 10 55.0 2.30549 7 0 7 56.0 2.30549 5 0 3 57.0 2.30549 8 0 8 58.0 2.30549 5 0 8 59.0 2.30549 7 0 6 60.0 2.30549 6 0 5 61.0 2.30549 5 0 5 62.0 2.30549 6 0 7 63.0 2.30549 6 0 4 64.0 2.30549 4 0 5 65.0 2.30549 7 0 8 66.0 2.30549 8 0 6 67.0 2.30549 8 0 10 68.0 2.30549 8 0 7 69.0 2.30549 5 0 5 70.0 2.30549 6 0 6 71.0 2.30549 4 0 4 72.0 2.30549 3 0 2 73.0 2.30549 1 1 1 74.0 2.30549 4 0 5 75.0 2.30549 2 0 2 76.0 2.30549 4 0 4 77.0 2.30549 3 0 2 78.0 2.30549 3 0 4 79.0 2.30549 4 0 3 80.0 2.30549 3 0 3 81.0 2.30549 4 0 4 82.0 2.30549 5 0 5 83.0 2.30549 2 0 2 84.0 2.30549 4 0 7 85.0 2.30549 4 0 1 86.0 2.30549 3 0 3 87.0 2.30549 2 0 3 88.0 2.30549 2 0 2 89.0 2.30549 5 0 5 90.0 2.30549 4 0 3 91.0 2.30549 3 0 6 92.0 2.30549 3 0 0 93.0 2.30549 3 0 4 94.0 2.30549 5 0 7 95.0 2.30549 0 0 0 96.0 2.30549 4 0 1 97.0 2.30549 3 0 3 98.0 2.30549 2 0 3 99.0 2.30549 4 0 4 100.0 2.30549 4 0 3 101.0 0 0 0 2 102.0 0 4 0 4 103.0 0 3 0 2 104.0 0 2 0 1 105.0 0 1 0 2 106.0 0 2 0 3 107.0 0 4 0 2 108.0 0 2 0 4 109.0 0 3 0 2 110.0 0 5 0 4 111.0 0 2 0 2 112.0 0 2 0 3 113.0 0 4 0 3 114.0 0 1 0 1 115.0 0 0 0 1 116.0 0 3 0 3 117.0 0 1 0 0 118.0 0 1 0 3 119.0 0 1 0 1 120.0 0 1 0 0 121.0 0 3 0 3 122.0 0 2 0 2 123.0 0 4 0 3 124.0 0 1 0 1 125.0 0 1 0 2 126.0 0 1 0 0 128.0 0 2 0 2 129.0 0 3 0 3 130.0 0 2 0 2 131.0 0 1 0 1 132.0 0 1 0 1 133.0 0 1 0 1 135.0 0 2 0 2 136.0 0 1 0 1 137.0 0 1 0 1 139.0 0 1 0 1 142.0 0 2 0 2 144.0 0 1 0 3 145.0 0 3 0 1 146.0 0 1 0 1 148.0 0 1 0 1 149.0 0 1 0 1 150.0 0 1 0 1 151.0 0 1 0 3 152.0 0 1 0 0 153.0 0 1 0 0 155.0 0 0 0 1 156.0 0 1 0 1 157.0 0 1 0 1 159.0 0 3 0 3 160.0 0 2 0 2 161.0 0 1 0 0 163.0 0 1 0 1 164.0 0 0 0 1 165.0 0 1 0 0 167.0 0 1 0 1 169.0 0 1 0 2 170.0 0 1 0 1 171.0 0 1 0 1 172.0 0 1 0 0 173.0 0 3 0 3 174.0 0 0 0 1 175.0 0 2 0 1 176.0 0 1 0 1 177.0 0 1 0 1 178.0 0 5 0 6 179.0 0 2 0 1 180.0 0 1 0 1 181.0 0 1 0 1 182.0 0 1 0 3 183.0 0 2 0 2 184.0 0 1 0 0 186.0 0 2 0 1 188.0 0 1 0 1 189.0 0 1 0 1 190.0 0 1 0 1 192.0 0 1 0 1 195.0 0 1 0 1 198.0 0 0 0 1 199.0 0 1 0 1 200.0 0 0 0 1 201.0 0 2 0 1 202.0 0 1 0 0 205.0 0 2 0 2 206.0 0 1 0 1 210.0 0 1 0 1 213.0 0 1 0 1 215.0 0 2 0 2 217.0 0 1 0 1 221.0 0 1 0 1 226.0 0 1 0 1 229.0 0 0 0 1 230.0 0 2 0 2 233.0 0 1 0 0 235.0 0 0 0 1 236.0 0 2 0 1 241.0 0 2 0 2 242.0 0 1 0 1 243.0 0 0 0 1 244.0 0 0 0 1 245.0 0 3 0 1 246.0 0 0 0 1 247.0 0 1 0 0 251.0 0 1 0 1 253.0 0 1 0 1 258.0 0 0 0 2 260.0 0 2 0 2 261.0 0 1 0 0 262.0 0 1 0 0 263.0 0 1 0 1 264.0 0 0 0 1 265.0 0 2 0 1 268.0 0 0 0 1 269.0 0 1 0 0 271.0 0 0 0 1 272.0 0 1 0 0 279.0 0 1 0 1 280.0 0 0 0 1 281.0 0 1 0 0 283.0 0 0 0 1 285.0 0 1 0 2 286.0 0 1 0 2 287.0 0 2 0 0 288.0 0 1 0 2 290.0 0 1 0 0 291.0 0 1 0 0 292.0 0 0 0 1 294.0 0 0 0 1 297.0 0 2 0 0 298.0 0 1 0 1 300.0 0 2 0 2 301.0 0 1 0 1 305.0 0 1 0 1 309.0 0 1 0 1 311.0 0 0 0 1 312.0 0 1 0 0 316.0 0 1 0 1 320.0 0 1 0 1 322.0 0 0 0 1 325.0 0 1 0 0 327.0 0 1 0 1 328.0 0 0 0 1 329.0 0 0 0 1 330.0 0 2 0 0 334.0 0 1 0 1 337.0 0 1 0 1 342.0 0 1 0 1 345.0 0 1 0 1 347.0 0 0 0 1 351.0 0 0 0 1 352.0 0 1 0 0 353.0 0 1 0 0 355.0 0 1 0 1 357.0 0 0 0 1 358.0 0 2 0 1 360.0 0 0 0 1 362.0 0 1 0 0 398.0 0 1 0 1 408.0 0 1 0 1 411.0 0 0 0 1 413.0 0 1 0 1 414.0 0 1 0 0 415.0 0 1 0 1 423.0 0 0 0 1 424.0 0 1 0 1 425.0 0 1 0 0 429.0 0 1 0 1 438.0 0 0 0 1 439.0 0 1 0 0 443.0 0 1 0 1 452.0 0 0 0 1 456.0 0 1 0 0 460.0 0 1 0 1 462.0 0 1 0 1 478.0 0 1 0 1 480.0 0 0 0 1 482.0 0 2 0 1 490.0 0 1 0 1 491.0 0 0 0 1 492.0 0 1 0 2 493.0 0 1 0 0 495.0 0 1 0 1 501.0 0 1 0 1 504.0 0 1 0 0 513.0 0 0 0 1 515.0 0 1 0 0 525.0 0 1 0 2 528.0 0 1 0 0 536.0 0 0 0 1 540.0 0 1 0 0 543.0 0 1 0 1 548.0 0 0 0 1 549.0 0 1 0 0 556.0 0 0 0 1 559.0 0 1 0 0 573.0 0 0 0 1 576.0 0 1 0 1 579.0 0 1 0 0 584.0 0 0 0 1 585.0 0 1 0 0 587.0 0 0 0 1 588.0 0 1 0 1 594.0 0 1 0 0 610.0 0 1 0 1 611.0 0 0 0 1 615.0 0 0 0 1 617.0 0 1 0 1 618.0 0 1 0 0 623.0 0 1 0 0 631.0 0 0 0 1 633.0 0 0 0 1 634.0 0 2 0 0 642.0 0 1 0 1 676.0 0 1 0 1 678.0 0 0 0 1 683.0 0 0 0 1 687.0 0 1 0 0 689.0 0 1 0 0 711.0 0 0 0 1 714.0 0 1 0 0 735.0 0 0 0 1 738.0 0 0 0 1 739.0 0 1 0 0 740.0 0 1 0 0 749.0 0 0 0 1 755.0 0 1 0 0 780.0 0 0 0 1 784.0 0 1 0 0 785.0 0 0 0 1 790.0 0 1 0 0 802.0 0 0 0 1 805.0 0 1 0 0 823.0 0 0 0 1 830.0 0 1 0 0 831.0 0 0 0 1 847.0 0 1 0 0 984.0 0 0 0 1 993.0 0 1 0 0 1050.0 0 0 0 1 1051.0 0 1 0 0 1106.0 0 1 0 1 1169.0 0 0 0 1 1175.0 0 1 0 0 1212.0 0 0 0 1 1217.0 0 1 0 0 1224.0 0 1 0 1 1226.0 0 0 0 1 1227.0 0 1 0 0 1271.0 0 0 0 1 1273.0 0 1 0 0 1288.0 0 0 0 1 1295.0 0 1 0 0 1325.0 0 0 0 1 1339.0 0 1 0 0 1346.0 0 0 0 1 1351.0 0 0 0 1 1355.0 0 1 0 0 1368.0 0 1 0 0 1392.0 0 0 0 1 1397.0 0 1 0 0 1430.0 0 0 0 1 1443.0 0 1 0 1 1444.0 0 1 0 0 1521.0 0 0 0 1 1532.0 0 1 0 1 1545.0 0 1 0 0 1558.0 0 0 0 1 1568.0 0 1 0 0 1724.0 0 0 0 1 1733.0 0 1 0 0 1841.0 0 0 0 1 1851.0 0 1 0 0 1855.0 0 0 0 1 1869.0 0 1 0 0 2173.0 0 0 0 1 2189.0 0 1 0 0 2249.0 0 0 0 1 2253.0 0 1 0 0 2734.0 0 0 0 1 2739.0 0 1 0 0 5644.0 0 0 0 1 5661.0 0 1 0 0 5953.0 0 0 0 1 5961.0 0 1 0 0 9157.0 0 0 0 1 9191.0 0 1 0 0 23260.0 0 0 0 1 23332.0 0 1 0 0