FastQCFastQC Report
Wed 21 Dec 2022
DE_Wue_23_Sep_2020.trimmed2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDE_Wue_23_Sep_2020.trimmed2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33647771
Sequences flagged as poor quality0
Sequence length75-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAACACAACACAACACAACACAACACAACACAACACAACACAACACA1493200.44377382382922187No Hit
GTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTT1401370.41648226861743676No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTT926770.2754328065297401No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACA879050.2612505892292241No Hit
CAACACAACACAACACAACACAACACAACACAACACAACACAACACAACA527970.15691083965116143No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT519300.15433414593792855No Hit
GTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGT495410.14723412139246905No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA420120.1248581964017765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)