## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/pipeline_output/DE_Mun_5_Sep_2020/DE_Mun_5_Sep_2020.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/pipeline_output/DE_Mun_5_Sep_2020/DE_Mun_5_Sep_2020.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/pipeline_output/DE_Mun_5_Sep_2020/DE_Mun_5_Sep_2020.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Wed Dec 21 03:48:16 UTC 2022 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 3648083 21928910 0 0 1939461 3982032 166715 0.208469 54576205 ## HISTOGRAM java.lang.Double BIN VALUE all_sets optical_sets non_optical_sets 1.0 1.006225 14495455 0 14607217 2.0 1.679503 2593086 164062 2522095 3.0 2.130001 457805 1241 427779 4.0 2.431435 79173 17 70854 5.0 2.633128 13987 2 12103 6.0 2.768083 2770 2 2343 7.0 2.858383 738 0 646 8.0 2.918804 342 0 332 9.0 2.959233 201 0 193 10.0 2.986284 170 0 175 11.0 3.004384 103 0 97 12.0 3.016495 86 0 86 13.0 3.024599 80 0 79 14.0 3.030021 50 0 50 15.0 3.033649 58 0 56 16.0 3.036077 54 1 57 17.0 3.037701 49 0 46 18.0 3.038788 36 0 35 19.0 3.039515 26 0 27 20.0 3.040002 23 0 23 21.0 3.040328 35 0 36 22.0 3.040545 24 0 23 23.0 3.040691 29 0 32 24.0 3.040789 28 0 28 25.0 3.040854 21 0 21 26.0 3.040898 18 0 14 27.0 3.040927 21 0 22 28.0 3.040946 13 0 14 29.0 3.04096 16 1 15 30.0 3.040968 14 0 14 31.0 3.040974 13 0 14 32.0 3.040978 11 0 9 33.0 3.040981 14 0 15 34.0 3.040982 10 0 9 35.0 3.040984 9 0 9 36.0 3.040984 8 0 9 37.0 3.040985 9 0 8 38.0 3.040985 9 0 9 39.0 3.040986 6 0 6 40.0 3.040986 10 0 10 41.0 3.040986 3 0 3 42.0 3.040986 7 0 7 43.0 3.040986 2 0 4 44.0 3.040986 14 0 12 45.0 3.040986 6 0 6 46.0 3.040986 7 0 7 47.0 3.040986 5 0 5 48.0 3.040986 11 0 11 49.0 3.040986 8 0 8 50.0 3.040986 7 0 7 51.0 3.040986 11 0 11 52.0 3.040986 7 0 8 53.0 3.040986 9 0 9 54.0 3.040986 9 0 9 55.0 3.040986 7 0 7 56.0 3.040986 10 0 9 57.0 3.040986 6 0 7 58.0 3.040986 4 0 5 59.0 3.040986 8 0 9 60.0 3.040986 5 1 3 61.0 3.040986 3 0 4 62.0 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