FastQCFastQC Report
Fri 20 Jan 2023
SRR3590550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45064071
Sequences flagged as poor quality0
Sequence length92
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2938120.6519872561003199No Hit
CTAGAGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG1752190.3888219508619184Illumina Single End PCR Primer 1 (100% over 42bp)
TGCACTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1442110.3200132540178183Illumina Single End PCR Primer 1 (100% over 43bp)
CAGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG1249490.2772696678913008Illumina Single End PCR Primer 1 (100% over 42bp)
GCTATCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1095840.24317376918743094Illumina Single End PCR Primer 1 (100% over 43bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1025750.22762035857790122Illumina Single End PCR Primer 1 (100% over 50bp)
CTGTAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT947250.21020071622024564Illumina Single End PCR Primer 1 (100% over 43bp)
CGTAGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT864870.19192007752695048Illumina Single End PCR Primer 1 (100% over 43bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC740020.1642150794587555Illumina Single End PCR Primer 1 (100% over 50bp)
AGTCTCGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG703800.156177634284306Illumina Single End PCR Primer 1 (100% over 42bp)
AGCTATCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG697160.1547041766377476Illumina Single End PCR Primer 1 (100% over 42bp)
GTCTCGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT614310.1363192419965786Illumina Single End PCR Primer 1 (100% over 43bp)
TGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT484470.10750693163074415Illumina Single End PCR Primer 1 (100% over 46bp)
TCGTGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT468020.10385657345515899Illumina Single End PCR Primer 1 (100% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)