FastQCFastQC Report
Fri 20 Jan 2023
SRR3590550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45064071
Sequences flagged as poor quality0
Sequence length92
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG2323460.5155903469085161Illumina Paired End PCR Primer 2 (100% over 43bp)
AGTCTCGAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTC1615770.35854949722584983Illumina Paired End PCR Primer 2 (100% over 42bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT1048200.23260215438591866Illumina Paired End PCR Primer 2 (100% over 50bp)
TGCACTGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG920120.20418039905893104Illumina Paired End PCR Primer 2 (100% over 43bp)
AGCTATCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTC809450.17962203192871767Illumina Paired End PCR Primer 2 (100% over 42bp)
GTCTCGAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG794570.17632006660028562Illumina Paired End PCR Primer 2 (100% over 43bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCG765380.1698426225185026Illumina Paired End PCR Primer 2 (100% over 50bp)
ATGCACTGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTC720130.15980136370724252Illumina Paired End PCR Primer 2 (100% over 42bp)
TCGTGCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG682790.1515153835080723Illumina Paired End PCR Primer 2 (100% over 43bp)
CTAGAGCTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTC580180.12874558093075966Illumina Paired End PCR Primer 2 (100% over 42bp)
AGCTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTAT535440.11881749431825632Illumina Paired End PCR Primer 2 (100% over 46bp)
GCTATCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG527130.11697345319733764Illumina Paired End PCR Primer 2 (100% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)