FastQCFastQC Report
Fri 20 Jan 2023
US_Pen_Sta_1_2014-06-18.trimmed2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUS_Pen_Sta_1_2014-06-18.trimmed2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102271236
Sequences flagged as poor quality0
Sequence length75-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT2074200.20281362395972216No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1750850.17119671849864024No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1496490.14632560028901967No Hit
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG1464570.14320448811237602No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA1462350.14298741828054176No Hit
ATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTT1319970.12906561528209162No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA1226140.11989099261497142No Hit
CTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATT1183010.11567377556676835No Hit
GTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTAT1179760.11535599315529932No Hit
CTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGT1114770.10900132271795367No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCT1088400.10642288512089557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)