FastQCFastQC Report
Fri 20 Jan 2023
US_Pen_Sta_1_2014-06-18.trimmed1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUS_Pen_Sta_1_2014-06-18.trimmed1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102271236
Sequences flagged as poor quality0
Sequence length75-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT2105030.20582815680451932No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1772490.17331266046300642No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1567220.15324152335462143No Hit
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG1516650.14829682903216304No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA1457550.14251807810360284No Hit
ATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTT1294690.1265937570168801No Hit
CTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGT1213710.11867559711510674No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA1181800.11555546273049834No Hit
GTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTAT1134240.11090508381066207No Hit
CTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATT1098640.1074241441650319No Hit
ATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAAC1089220.10650306406778931No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCT1048130.10248531659478527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)