## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/pipeline_output//PA_li_15_spring/PA_li_15_spring.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/pipeline_output/PA_li_15_spring/PA_li_15_spring.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/pipeline_output/PA_li_15_spring/PA_li_15_spring.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Thu Jun 11 08:23:18 UTC 2020 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 3148711 123540413 0 0 2180785 24496037 0 0.204504 268817280 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 76732690 76732690 2.0 1.631555 16270700 16270700 3.0 2.030417 2902677 2902677 4.0 2.28232 478951 478951 5.0 2.441411 84906 84906 6.0 2.541885 21130 21130 7.0 2.60534 8653 8653 8.0 2.645416 4851 4851 9.0 2.670726 3255 3255 10.0 2.68671 2215 2215 11.0 2.696805 1646 1646 12.0 2.703181 1235 1235 13.0 2.707208 926 926 14.0 2.709751 786 786 15.0 2.711357 610 610 16.0 2.712371 512 512 17.0 2.713011 403 403 18.0 2.713416 379 379 19.0 2.713672 317 317 20.0 2.713833 292 292 21.0 2.713935 261 261 22.0 2.713999 226 226 23.0 2.71404 207 207 24.0 2.714065 206 206 25.0 2.714082 194 194 26.0 2.714092 154 154 27.0 2.714098 137 137 28.0 2.714102 125 125 29.0 2.714105 131 131 30.0 2.714107 96 96 31.0 2.714108 112 112 32.0 2.714108 97 97 33.0 2.714109 79 79 34.0 2.714109 65 65 35.0 2.714109 85 85 36.0 2.714109 70 70 37.0 2.714109 77 77 38.0 2.714109 55 55 39.0 2.714109 60 60 40.0 2.714109 52 52 41.0 2.714109 63 63 42.0 2.714109 56 56 43.0 2.714109 53 53 44.0 2.714109 44 44 45.0 2.714109 48 48 46.0 2.714109 41 41 47.0 2.714109 46 46 48.0 2.714109 54 54 49.0 2.714109 44 44 50.0 2.714109 28 28 51.0 2.714109 24 24 52.0 2.714109 38 38 53.0 2.714109 25 25 54.0 2.714109 41 41 55.0 2.714109 37 37 56.0 2.714109 35 35 57.0 2.714109 24 24 58.0 2.714109 19 19 59.0 2.714109 27 27 60.0 2.714109 31 31 61.0 2.714109 21 21 62.0 2.714109 24 24 63.0 2.714109 27 27 64.0 2.714109 22 22 65.0 2.714109 14 14 66.0 2.714109 18 18 67.0 2.714109 12 12 68.0 2.714109 17 17 69.0 2.714109 16 16 70.0 2.714109 19 19 71.0 2.714109 17 17 72.0 2.714109 17 17 73.0 2.714109 10 10 74.0 2.714109 16 16 75.0 2.714109 13 13 76.0 2.714109 16 16 77.0 2.714109 14 14 78.0 2.714109 14 14 79.0 2.714109 18 18 80.0 2.714109 15 15 81.0 2.714109 12 12 82.0 2.714109 12 12 83.0 2.714109 20 20 84.0 2.714109 18 18 85.0 2.714109 13 13 86.0 2.714109 10 10 87.0 2.714109 6 6 88.0 2.714109 11 11 89.0 2.714109 14 14 90.0 2.714109 6 6 91.0 2.714109 10 10 92.0 2.714109 11 11 93.0 2.714109 8 8 94.0 2.714109 7 7 95.0 2.714109 4 4 96.0 2.714109 14 14 97.0 2.714109 11 11 98.0 2.714109 5 5 99.0 2.714109 10 10 100.0 2.714109 9 9 101.0 0 6 6 102.0 0 13 13 103.0 0 12 12 104.0 0 10 10 105.0 0 10 10 106.0 0 8 8 107.0 0 6 6 108.0 0 5 5 109.0 0 5 5 110.0 0 10 10 111.0 0 9 9 112.0 0 5 5 113.0 0 7 7 114.0 0 6 6 115.0 0 6 6 116.0 0 7 7 118.0 0 6 6 119.0 0 6 6 120.0 0 6 6 121.0 0 8 8 122.0 0 6 6 123.0 0 4 4 124.0 0 3 3 125.0 0 6 6 126.0 0 3 3 127.0 0 2 2 128.0 0 3 3 129.0 0 7 7 130.0 0 8 8 131.0 0 6 6 132.0 0 5 5 133.0 0 14 14 134.0 0 3 3 135.0 0 8 8 136.0 0 8 8 137.0 0 1 1 138.0 0 2 2 139.0 0 2 2 140.0 0 2 2 141.0 0 5 5 142.0 0 5 5 143.0 0 12 12 144.0 0 3 3 145.0 0 4 4 146.0 0 4 4 147.0 0 3 3 148.0 0 3 3 149.0 0 2 2 150.0 0 6 6 151.0 0 3 3 153.0 0 2 2 154.0 0 6 6 155.0 0 2 2 156.0 0 3 3 157.0 0 2 2 158.0 0 5 5 159.0 0 4 4 160.0 0 5 5 161.0 0 3 3 162.0 0 3 3 163.0 0 2 2 164.0 0 5 5 165.0 0 3 3 166.0 0 1 1 167.0 0 2 2 168.0 0 4 4 169.0 0 3 3 170.0 0 2 2 171.0 0 1 1 172.0 0 1 1 173.0 0 1 1 174.0 0 1 1 175.0 0 4 4 176.0 0 2 2 177.0 0 1 1 178.0 0 5 5 180.0 0 5 5 182.0 0 1 1 183.0 0 1 1 184.0 0 4 4 185.0 0 4 4 186.0 0 2 2 188.0 0 3 3 189.0 0 2 2 190.0 0 3 3 191.0 0 2 2 192.0 0 2 2 194.0 0 5 5 196.0 0 1 1 197.0 0 1 1 198.0 0 1 1 200.0 0 1 1 201.0 0 1 1 202.0 0 2 2 203.0 0 3 3 204.0 0 3 3 205.0 0 1 1 206.0 0 1 1 207.0 0 1 1 208.0 0 1 1 209.0 0 1 1 210.0 0 1 1 212.0 0 3 3 215.0 0 1 1 216.0 0 2 2 217.0 0 2 2 219.0 0 3 3 220.0 0 2 2 221.0 0 2 2 223.0 0 1 1 224.0 0 1 1 225.0 0 1 1 226.0 0 1 1 227.0 0 4 4 228.0 0 1 1 229.0 0 1 1 230.0 0 1 1 231.0 0 2 2 233.0 0 1 1 234.0 0 1 1 235.0 0 1 1 236.0 0 2 2 237.0 0 1 1 238.0 0 1 1 240.0 0 1 1 241.0 0 2 2 242.0 0 3 3 243.0 0 5 5 244.0 0 1 1 245.0 0 2 2 247.0 0 1 1 249.0 0 1 1 252.0 0 2 2 253.0 0 3 3 254.0 0 1 1 256.0 0 2 2 257.0 0 1 1 258.0 0 1 1 261.0 0 3 3 262.0 0 4 4 263.0 0 1 1 264.0 0 3 3 265.0 0 2 2 266.0 0 1 1 267.0 0 1 1 270.0 0 2 2 271.0 0 1 1 273.0 0 1 1 274.0 0 1 1 276.0 0 1 1 277.0 0 1 1 278.0 0 2 2 282.0 0 3 3 286.0 0 3 3 289.0 0 1 1 291.0 0 1 1 292.0 0 1 1 293.0 0 1 1 294.0 0 2 2 296.0 0 2 2 297.0 0 1 1 301.0 0 2 2 303.0 0 1 1 304.0 0 1 1 305.0 0 2 2 306.0 0 1 1 307.0 0 1 1 309.0 0 1 1 311.0 0 2 2 312.0 0 1 1 314.0 0 1 1 317.0 0 1 1 321.0 0 2 2 322.0 0 1 1 323.0 0 1 1 332.0 0 1 1 339.0 0 1 1 341.0 0 2 2 342.0 0 1 1 346.0 0 1 1 348.0 0 1 1 349.0 0 1 1 356.0 0 1 1 359.0 0 1 1 361.0 0 1 1 363.0 0 1 1 365.0 0 1 1 367.0 0 1 1 375.0 0 1 1 376.0 0 1 1 378.0 0 1 1 380.0 0 1 1 381.0 0 1 1 382.0 0 1 1 383.0 0 1 1 386.0 0 1 1 389.0 0 1 1 390.0 0 1 1 391.0 0 1 1 392.0 0 1 1 394.0 0 2 2 395.0 0 1 1 397.0 0 1 1 399.0 0 1 1 401.0 0 1 1 402.0 0 1 1 403.0 0 1 1 408.0 0 1 1 409.0 0 1 1 410.0 0 1 1 414.0 0 2 2 417.0 0 1 1 420.0 0 4 4 421.0 0 1 1 423.0 0 1 1 424.0 0 1 1 427.0 0 1 1 429.0 0 1 1 432.0 0 2 2 434.0 0 1 1 436.0 0 2 2 439.0 0 1 1 442.0 0 1 1 444.0 0 1 1 451.0 0 2 2 452.0 0 1 1 456.0 0 1 1 461.0 0 1 1 473.0 0 2 2 475.0 0 1 1 476.0 0 1 1 478.0 0 1 1 480.0 0 1 1 483.0 0 1 1 493.0 0 1 1 497.0 0 1 1 500.0 0 1 1 512.0 0 1 1 513.0 0 1 1 519.0 0 1 1 520.0 0 1 1 524.0 0 1 1 527.0 0 1 1 531.0 0 1 1 538.0 0 2 2 546.0 0 1 1 548.0 0 1 1 549.0 0 1 1 556.0 0 1 1 558.0 0 1 1 562.0 0 1 1 563.0 0 1 1 564.0 0 1 1 570.0 0 1 1 572.0 0 1 1 573.0 0 1 1 574.0 0 1 1 576.0 0 2 2 580.0 0 1 1 581.0 0 1 1 585.0 0 1 1 586.0 0 1 1 588.0 0 1 1 592.0 0 1 1 598.0 0 1 1 605.0 0 1 1 606.0 0 1 1 630.0 0 1 1 651.0 0 1 1 657.0 0 1 1 658.0 0 1 1 663.0 0 1 1 666.0 0 1 1 686.0 0 1 1 687.0 0 1 1 688.0 0 1 1 689.0 0 1 1 693.0 0 1 1 701.0 0 1 1 703.0 0 1 1 719.0 0 1 1 727.0 0 1 1 739.0 0 1 1 781.0 0 2 2 787.0 0 1 1 800.0 0 1 1 802.0 0 1 1 810.0 0 1 1 814.0 0 1 1 830.0 0 1 1 836.0 0 1 1 844.0 0 1 1 852.0 0 1 1 860.0 0 1 1 882.0 0 1 1 935.0 0 1 1 988.0 0 1 1 1010.0 0 1 1 1030.0 0 1 1 1047.0 0 1 1 1121.0 0 1 1 1132.0 0 1 1 1161.0 0 1 1 1202.0 0 1 1 1287.0 0 1 1