FastQCFastQC Report
Thu 11 Jun 2020
PA_li_15_spring.trimmed1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePA_li_15_spring.trimmed1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160991468
Sequences flagged as poor quality0
Sequence length75-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1954800.12142258371108214No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1874150.11641300146415212No Hit
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG1872420.11630554235333765No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA1762970.10950704542926461No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1640680.101910990711632No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)