## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/US_Pen_Lin_1_2009-11-16/US_Pen_Lin_1_2009-11-16.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Pen_Lin_1_2009-11-16/US_Pen_Lin_1_2009-11-16.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Pen_Lin_1_2009-11-16/US_Pen_Lin_1_2009-11-16.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 18:45:39 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 1016745 42572128 0 0 344768 985518 0 0.026878 905263229 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 39630327 39630327 2.0 1.954061 338367 338367 3.0 2.864294 28889 28889 4.0 3.732712 5779 5779 5.0 4.561236 2340 2340 6.0 5.351699 1324 1324 7.0 6.105849 890 890 8.0 6.825354 596 596 9.0 7.511807 421 421 10.0 8.166724 347 347 11.0 8.791555 290 290 12.0 9.387683 231 231 13.0 9.956425 190 190 14.0 10.49904 148 148 15.0 11.016728 133 133 16.0 11.510633 117 117 17.0 11.98185 101 101 18.0 12.431419 90 90 19.0 12.860336 102 102 20.0 13.269549 87 87 21.0 13.659964 81 81 22.0 14.032443 84 84 23.0 14.387811 57 57 24.0 14.726853 79 79 25.0 15.050321 47 47 26.0 15.358929 44 44 27.0 15.65336 53 53 28.0 15.934265 55 55 29.0 16.202266 38 38 30.0 16.457955 29 29 31.0 16.701898 37 37 32.0 16.934634 33 33 33.0 17.156679 26 26 34.0 17.368524 25 25 35.0 17.570637 27 27 36.0 17.763465 23 23 37.0 17.947434 33 33 38.0 18.122952 24 24 39.0 18.290408 18 18 40.0 18.45017 20 20 41.0 18.602594 21 21 42.0 18.748015 17 17 43.0 18.886755 13 13 44.0 19.019123 19 19 45.0 19.145409 9 9 46.0 19.265894 14 14 47.0 19.380844 12 12 48.0 19.490513 14 14 49.0 19.595145 14 14 50.0 19.694969 18 18 51.0 19.790208 11 11 52.0 19.881072 12 12 53.0 19.967761 8 8 54.0 20.050468 9 9 55.0 20.129376 12 12 56.0 20.204659 5 5 57.0 20.276483 10 10 58.0 20.345008 10 10 59.0 20.410385 13 13 60.0 20.472759 7 7 61.0 20.532267 12 12 62.0 20.589041 8 8 63.0 20.643208 7 7 64.0 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