FastQCFastQC Report
Fri 20 Jan 2023
SRR3590558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43400925
Sequences flagged as poor quality0
Sequence length92
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGAGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG3053800.7036255563677503Illumina Single End PCR Primer 1 (100% over 42bp)
TGCACTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT3049320.7025933203036571Illumina Single End PCR Primer 1 (100% over 43bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2490000.573720490980319No Hit
CTGTAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT2434310.560888967228233Illumina Single End PCR Primer 1 (100% over 43bp)
CAGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG2218690.5112079984470377Illumina Single End PCR Primer 1 (100% over 42bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2115500.48743200749753607Illumina Single End PCR Primer 1 (100% over 50bp)
GCTATCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT2005000.46197172064881104Illumina Single End PCR Primer 1 (100% over 43bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1254260.2889938405690662Illumina Single End PCR Primer 1 (100% over 50bp)
TCGTGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1138720.2623722881482365Illumina Single End PCR Primer 1 (100% over 43bp)
TGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT1028000.23686131113564976Illumina Single End PCR Primer 1 (100% over 46bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)