FastQCFastQC Report
Fri 20 Jan 2023
SRR3590558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43400925
Sequences flagged as poor quality0
Sequence length92
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG4926091.1350195877161604Illumina Paired End PCR Primer 2 (100% over 43bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT2176210.501420188625012Illumina Paired End PCR Primer 2 (100% over 50bp)
TGCACTGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1995090.45968835917667655Illumina Paired End PCR Primer 2 (100% over 43bp)
GTCTCGAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1675210.38598486092174306Illumina Paired End PCR Primer 2 (100% over 43bp)
TCGTGCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1464730.3374881986962259Illumina Paired End PCR Primer 2 (100% over 43bp)
ATGCACTGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTC1316340.30329768317149924Illumina Paired End PCR Primer 2 (100% over 42bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCG1304810.3006410577654739Illumina Paired End PCR Primer 2 (100% over 50bp)
GCTATCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1158920.26702656683008485Illumina Paired End PCR Primer 2 (100% over 43bp)
AGCTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTAT1138720.2623722881482365Illumina Paired End PCR Primer 2 (100% over 46bp)
CGTAGCTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG978630.22548597754540947Illumina Paired End PCR Primer 2 (100% over 43bp)
ATGACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA736140.16961389647801284Illumina Paired End PCR Primer 2 (100% over 45bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)