## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/US_Flo_Hom_1_2010-07-15/US_Flo_Hom_1_2010-07-15.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Flo_Hom_1_2010-07-15/US_Flo_Hom_1_2010-07-15.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Flo_Hom_1_2010-07-15/US_Flo_Hom_1_2010-07-15.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 17:39:06 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 3729571 38182947 0 0 1237800 644136 0 0.031538 1118936254 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 36566595 36566595 2.0 1.966451 233272 233272 3.0 2.90048 18560 18560 4.0 3.803172 4908 4908 5.0 4.675581 2085 2085 6.0 5.518722 1109 1109 7.0 6.333576 709 709 8.0 7.121093 464 464 9.0 7.88219 303 303 10.0 8.617753 264 264 11.0 9.328639 179 179 12.0 10.015675 166 166 13.0 10.679663 111 111 14.0 11.321375 77 77 15.0 11.941558 100 100 16.0 12.540934 67 67 17.0 13.120203 61 61 18.0 13.680038 61 61 19.0 14.221091 53 53 20.0 14.743992 48 48 21.0 15.249351 49 49 22.0 15.737756 36 36 23.0 16.209775 34 34 24.0 16.665959 31 31 25.0 17.106838 28 28 26.0 17.532927 34 34 27.0 17.944721 26 26 28.0 18.342699 28 28 29.0 18.727326 29 29 30.0 19.09905 19 19 31.0 19.458302 25 25 32.0 19.805502 15 15 33.0 20.141054 17 17 34.0 20.465349 7 7 35.0 20.778764 5 5 36.0 21.081664 11 11 37.0 21.374403 10 10 38.0 21.65732 14 14 39.0 21.930746 15 15 40.0 22.194999 10 10 41.0 22.450386 10 10 42.0 22.697206 12 12 43.0 22.935745 9 9 44.0 23.166282 12 12 45.0 23.389084 9 9 46.0 23.604412 12 12 47.0 23.812515 3 3 48.0 24.013637 7 7 49.0 24.208012 11 11 50.0 24.395866 14 14 51.0 24.577417 9 9 52.0 24.752878 7 7 53.0 24.922452 9 9 54.0 25.086337 6 6 55.0 25.244724 5 5 56.0 25.397797 12 12 57.0 25.545735 3 3 58.0 25.68871 7 7 59.0 25.826888 4 4 60.0 25.960431 7 7 61.0 26.089493 7 7 62.0 26.214226 6 6 63.0 26.334774 6 6 64.0 26.451277 4 4 65.0 26.563872 5 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