## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Tuo_1_2013-12-08/US_Cal_Tuo_1_2013-12-08.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Tuo_1_2013-12-08/US_Cal_Tuo_1_2013-12-08.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Tuo_1_2013-12-08/US_Cal_Tuo_1_2013-12-08.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 17:39:38 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 1582675 29624869 0 0 463819 1733139 0 0.064605 243217344 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 26238284 26238284 2.0 1.885322 1097088 1097088 3.0 2.669116 42142 42142 4.0 3.363027 3655 3655 5.0 3.977361 1382 1382 6.0 4.521244 965 965 7.0 5.002756 646 646 8.0 5.429049 480 480 9.0 5.806455 396 396 10.0 6.140581 283 283 11.0 6.436391 253 253 12.0 6.698277 210 210 13.0 6.93013 180 180 14.0 7.135395 173 173 15.0 7.317121 137 137 16.0 7.478007 114 114 17.0 7.620442 122 122 18.0 7.746544 99 99 19.0 7.858184 115 115 20.0 7.957021 86 86 21.0 8.044524 85 85 22.0 8.121993 73 73 23.0 8.190577 64 64 24.0 8.251296 65 65 25.0 8.305052 75 75 26.0 8.352644 58 58 27.0 8.394778 39 39 28.0 8.43208 53 53 29.0 8.465104 51 51 30.0 8.494341 51 51 31.0 8.520225 48 48 32.0 8.543141 42 42 33.0 8.563429 37 37 34.0 8.58139 39 39 35.0 8.597292 43 43 36.0 8.61137 30 30 37.0 8.623833 24 24 38.0 8.634867 26 26 39.0 8.644636 30 30 40.0 8.653285 40 40 41.0 8.660942 30 30 42.0 8.66772 28 28 43.0 8.673722 28 28 44.0 8.679035 31 31 45.0 8.683739 24 24 46.0 8.687903 23 23 47.0 8.69159 22 22 48.0 8.694854 23 23 49.0 8.697744 12 12 50.0 8.700302 19 19 51.0 8.702567 20 20 52.0 8.704572 25 25 53.0 8.706347 18 18 54.0 8.707919 17 17 55.0 8.70931 12 12 56.0 8.710542 14 14 57.0 8.711633 23 23 58.0 8.712598 12 12 59.0 8.713453 10 10 60.0 8.71421 24 24 61.0 8.71488 17 17 62.0 8.715473 17 17 63.0 8.715998 17 17 64.0 8.716463 13 13 65.0 8.716875 16 16 66.0 8.717239 12 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