FastQCFastQC Report
Fri 20 Jan 2023
US_Cal_Tuo_1_2012-10-10.trimmed2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUS_Cal_Tuo_1_2012-10-10.trimmed2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56197178
Sequences flagged as poor quality0
Sequence length75-92
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATT2381200.42372234420739063No Hit
ATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAAC1812610.3225446658549296No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1516060.2697751121951355No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCT1437060.2557174668094544No Hit
ATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTT1328260.2363570640504404No Hit
AGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACAT1313760.2337768633150939No Hit
CATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAA1173620.20883966807016535No Hit
AATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAG1147310.20415793832209866No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA1131220.2012948052302555No Hit
ATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGA1010580.17982753511217234No Hit
AACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAAT802920.14287550168444402No Hit
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG787270.14009066433905276No Hit
ACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATA760420.13531284435670418No Hit
GTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTAT721460.1283801118981455No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT712200.1267323423250897No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT698320.12426246741428904No Hit
TATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTC680060.12101319393653538No Hit
TGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTA671790.11954158979299637No Hit
TTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTA582150.1035906109022058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)