FastQCFastQC Report
Fri 20 Jan 2023
US_Cal_Tuo_1_2012-10-10.trimmed1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUS_Cal_Tuo_1_2012-10-10.trimmed1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56197178
Sequences flagged as poor quality0
Sequence length75-92
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATT2326830.4140474811742326No Hit
ATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAAC1795090.3194270715871178No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCT1420710.25280806804925327No Hit
ATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTT1282500.22821430641944335No Hit
AGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACAT1237450.22019788965203912No Hit
CATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAA1161150.20662069543776734No Hit
AATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAG1156640.20581816403663541No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1128500.20081079516128014No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1052980.18737239795208221No Hit
ATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGA1013820.18040407651786358No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG985320.1753326474863204No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA803610.14299828365047085No Hit
AACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAAT771640.1373093858912275No Hit
ACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATA761170.13544630301542898No Hit
TATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTC698150.12423221678497806No Hit
TGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTA694650.1236094097109289No Hit
GTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTAT664780.11829419619611503No Hit
TTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTA596890.10621351840834428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)