## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Sto_1_2012-11-15/US_Cal_Sto_1_2012-11-15.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Sto_1_2012-11-15/US_Cal_Sto_1_2012-11-15.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Sto_1_2012-11-15/US_Cal_Sto_1_2012-11-15.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 17:45:55 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 3337969 26184703 0 0 911703 2105364 0 0.091952 153980884 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 22249564 22249564 2.0 1.843621 1258250 1258250 3.0 2.555318 62512 62512 4.0 3.155721 5505 5505 5.0 3.662233 1884 1884 6.0 4.089538 1180 1180 7.0 4.450021 753 753 8.0 4.754132 580 580 9.0 5.010687 433 433 10.0 5.227122 337 337 11.0 5.409711 277 277 12.0 5.563747 222 222 13.0 5.693695 206 206 14.0 5.803322 189 189 15.0 5.895806 158 158 16.0 5.973827 144 144 17.0 6.039648 115 115 18.0 6.095175 89 89 19.0 6.142019 85 85 20.0 6.181538 85 85 21.0 6.214877 84 84 22.0 6.243002 78 78 23.0 6.266729 81 81 24.0 6.286746 64 64 25.0 6.303632 76 76 26.0 6.317878 53 53 27.0 6.329896 59 59 28.0 6.340035 59 59 29.0 6.348588 58 58 30.0 6.355804 63 63 31.0 6.361891 42 42 32.0 6.367027 47 47 33.0 6.371359 48 48 34.0 6.375014 39 39 35.0 6.378097 41 41 36.0 6.380698 39 39 37.0 6.382892 33 33 38.0 6.384744 39 39 39.0 6.386305 26 26 40.0 6.387623 22 22 41.0 6.388734 36 36 42.0 6.389672 24 24 43.0 6.390463 20 20 44.0 6.39113 27 27 45.0 6.391693 21 21 46.0 6.392168 21 21 47.0 6.392569 29 29 48.0 6.392907 19 19 49.0 6.393192 18 18 50.0 6.393433 17 17 51.0 6.393636 20 20 52.0 6.393807 18 18 53.0 6.393951 16 16 54.0 6.394073 15 15 55.0 6.394176 19 19 56.0 6.394263 13 13 57.0 6.394336 11 11 58.0 6.394398 14 14 59.0 6.39445 17 17 60.0 6.394494 18 18 61.0 6.394531 10 10 62.0 6.394562 12 12 63.0 6.394588 14 14 64.0 6.39461 9 9 65.0 6.394629 14 14 66.0 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