FastQCFastQC Report
Fri 20 Jan 2023
SRR3939081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3939081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60845162
Sequences flagged as poor quality0
Sequence length92
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG1862440.30609500226164243No Hit
CAGCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1760180.2892884071867538Illumina Single End PCR Primer 1 (100% over 43bp)
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC1682750.2765626624512891No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1672170.2748238224758116No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA1271190.20892211610842618No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1133930.1863632148764761No Hit
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC1115460.18332764074159258No Hit
CGACGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT996060.16370405916578873Illumina Single End PCR Primer 1 (100% over 43bp)
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT854750.14047953393566442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)