## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Pla_1_2013-12-16/US_Cal_Pla_1_2013-12-16.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Pla_1_2013-12-16/US_Cal_Pla_1_2013-12-16.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Pla_1_2013-12-16/US_Cal_Pla_1_2013-12-16.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 17:38:31 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 3390865 31021875 0 0 1059903 1652778 0 0.066715 280697937 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 27765707 27765707 2.0 1.895371 1104319 1104319 3.0 2.697061 39666 39666 4.0 3.41487 3067 3067 5.0 4.057576 1155 1155 6.0 4.633037 763 763 7.0 5.148288 604 604 8.0 5.609628 413 413 9.0 6.0227 322 322 10.0 6.392552 275 275 11.0 6.723706 236 236 12.0 7.020213 183 183 13.0 7.285696 150 150 14.0 7.523402 136 136 15.0 7.736238 131 131 16.0 7.926804 95 95 17.0 8.097432 101 101 18.0 8.250207 93 93 19.0 8.386997 69 69 20.0 8.509476 79 79 21.0 8.619139 72 72 22.0 8.717329 67 67 23.0 8.805245 54 54 24.0 8.883962 61 61 25.0 8.954444 50 50 26.0 9.017551 73 73 27.0 9.074055 62 62 28.0 9.124647 51 51 29.0 9.169946 43 43 30.0 9.210505 45 45 31.0 9.246821 31 31 32.0 9.279337 33 33 33.0 9.308451 32 32 34.0 9.334518 37 37 35.0 9.357859 23 23 36.0 9.378757 30 30 37.0 9.397468 28 28 38.0 9.414222 24 24 39.0 9.429223 26 26 40.0 9.442655 23 23 41.0 9.454681 24 24 42.0 9.465449 29 29 43.0 9.47509 27 27 44.0 9.483722 20 20 45.0 9.491451 21 21 46.0 9.498372 24 24 47.0 9.504568 18 18 48.0 9.510117 21 21 49.0 9.515084 21 21 50.0 9.519532 20 20 51.0 9.523514 13 13 52.0 9.52708 19 19 53.0 9.530273 25 25 54.0 9.533132 20 20 55.0 9.535691 12 12 56.0 9.537983 12 12 57.0 9.540035 19 19 58.0 9.541872 15 15 59.0 9.543517 12 12 60.0 9.54499 11 11 61.0 9.546309 13 13 62.0 9.54749 10 10 63.0 9.548547 11 11 64.0 9.549494 10 10 65.0 9.550342 11 11 66.0 9.5511 12 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