## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Pla_1_2013-08-30/US_Cal_Pla_1_2013-08-30.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Pla_1_2013-08-30/US_Cal_Pla_1_2013-08-30.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Pla_1_2013-08-30/US_Cal_Pla_1_2013-08-30.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 18:12:33 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 3361447 41225535 0 0 1168979 1408329 0 0.046446 589568975 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 37842196 37842196 2.0 1.932464 919824 919824 3.0 2.801953 35210 35210 4.0 3.61272 6814 6814 5.0 4.368731 3210 3210 6.0 5.073683 1720 1720 7.0 5.731027 1088 1088 8.0 6.343975 776 776 9.0 6.915528 510 510 10.0 7.44848 400 400 11.0 7.945438 331 331 12.0 8.408834 291 291 13.0 8.840934 213 213 14.0 9.243852 197 197 15.0 9.619558 189 189 16.0 9.96989 143 143 17.0 10.296562 119 119 18.0 10.601172 126 126 19.0 10.88521 125 125 20.0 11.150065 92 92 21.0 11.397033 82 82 22.0 11.627321 60 60 23.0 11.842057 68 68 24.0 12.04229 58 58 25.0 12.229001 55 55 26.0 12.403101 61 61 27.0 12.565444 62 62 28.0 12.716823 44 44 29.0 12.857978 44 44 30.0 12.9896 53 53 31.0 13.112332 40 40 32.0 13.226776 38 38 33.0 13.333491 39 39 34.0 13.432999 33 33 35.0 13.525786 40 40 36.0 13.612307 34 34 37.0 13.692984 38 38 38.0 13.768213 25 25 39.0 13.838361 29 29 40.0 13.903772 27 27 41.0 13.964765 22 22 42.0 14.021639 24 24 43.0 14.074672 28 28 44.0 14.124123 26 26 45.0 14.170234 31 31 46.0 14.213231 22 22 47.0 14.253325 29 29 48.0 14.29071 23 23 49.0 14.325571 18 18 50.0 14.358078 20 20 51.0 14.388389 16 16 52.0 14.416653 16 16 53.0 14.443008 20 20 54.0 14.467583 21 21 55.0 14.490498 16 16 56.0 14.511866 13 13 57.0 14.531791 18 18 58.0 14.55037 14 14 59.0 14.567694 18 18 60.0 14.583849 11 11 61.0 14.598912 13 13 62.0 14.612958 13 13 63.0 14.626055 12 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