## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Esp_1_2013-09-01/US_Cal_Esp_1_2013-09-01.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Esp_1_2013-09-01/US_Cal_Esp_1_2013-09-01.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Esp_1_2013-09-01/US_Cal_Esp_1_2013-09-01.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 18:25:03 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 3512590 42520163 0 0 1259805 2235608 0 0.064719 390055080 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 37363751 37363751 2.0 1.896721 1440272 1440272 3.0 2.700829 77386 77386 4.0 3.42189 14620 14620 5.0 4.06848 6141 6141 6.0 4.648291 3398 3398 7.0 5.168219 2051 2051 8.0 5.63445 1299 1299 9.0 6.052529 932 932 10.0 6.427429 691 691 11.0 6.76361 552 552 12.0 7.06507 428 428 13.0 7.335395 353 353 14.0 7.577802 314 314 15.0 7.795173 243 243 16.0 7.990094 225 225 17.0 8.164884 185 185 18.0 8.321622 165 165 19.0 8.462172 177 177 20.0 8.588206 140 140 21.0 8.701223 116 116 22.0 8.802569 130 130 23.0 8.893447 105 105 24.0 8.974939 113 113 25.0 9.048015 104 104 26.0 9.113544 78 78 27.0 9.172305 81 81 28.0 9.224997 73 73 29.0 9.272247 61 61 30.0 9.314618 73 73 31.0 9.352612 60 60 32.0 9.386682 66 66 33.0 9.417234 57 57 34.0 9.44463 45 45 35.0 9.469196 56 56 36.0 9.491226 51 51 37.0 9.51098 52 52 38.0 9.528694 37 37 39.0 9.544579 46 46 40.0 9.558823 43 43 41.0 9.571596 30 30 42.0 9.58305 42 42 43.0 9.593321 30 30 44.0 9.602531 34 34 45.0 9.610789 30 30 46.0 9.618195 19 19 47.0 9.624836 23 23 48.0 9.630791 30 30 49.0 9.636132 28 28 50.0 9.64092 24 24 51.0 9.645214 24 24 52.0 9.649065 20 20 53.0 9.652518 22 22 54.0 9.655614 14 14 55.0 9.65839 30 30 56.0 9.66088 17 17 57.0 9.663113 28 28 58.0 9.665115 29 29 59.0 9.66691 20 20 60.0 9.66852 20 20 61.0 9.669963 18 18 62.0 9.671258 13 13 63.0 9.672418 19 19 64.0 9.673459 18 18 65.0 9.674393 26 26 66.0 9.67523 18 18 67.0 9.67598 16 16 68.0 9.676653 16 16 69.0 9.677257 9 9 70.0 9.677798 12 12 71.0 9.678283 16 16 72.0 9.678719 9 9 73.0 9.679109 7 7 74.0 9.679459 9 9 75.0 9.679772 13 13 76.0 9.680054 14 14 77.0 9.680306 10 10 78.0 9.680532 12 12 79.0 9.680735 6 6 80.0 9.680917 6 6 81.0 9.68108 10 10 82.0 9.681227 14 14 83.0 9.681358 12 12 84.0 9.681476 11 11 85.0 9.681581 16 16 86.0 9.681676 13 13 87.0 9.68176 12 12 88.0 9.681836 9 9 89.0 9.681905 10 10 90.0 9.681966 13 13 91.0 9.682021 8 8 92.0 9.68207 7 7 93.0 9.682114 6 6 94.0 9.682154 11 11 95.0 9.682189 9 9 96.0 9.682221 6 6 97.0 9.682249 5 5 98.0 9.682275 4 4 99.0 9.682298 9 9 100.0 9.682318 9 9 101.0 0 3 3 102.0 0 9 9 103.0 0 8 8 104.0 0 3 3 105.0 0 5 5 106.0 0 2 2 107.0 0 5 5 108.0 0 6 6 109.0 0 6 6 110.0 0 6 6 111.0 0 12 12 112.0 0 7 7 113.0 0 9 9 114.0 0 8 8 115.0 0 3 3 116.0 0 10 10 117.0 0 5 5 118.0 0 5 5 119.0 0 4 4 120.0 0 6 6 121.0 0 9 9 122.0 0 6 6 123.0 0 4 4 124.0 0 6 6 125.0 0 9 9 126.0 0 4 4 127.0 0 2 2 128.0 0 4 4 129.0 0 6 6 130.0 0 10 10 131.0 0 3 3 132.0 0 9 9 133.0 0 8 8 134.0 0 6 6 135.0 0 3 3 136.0 0 5 5 137.0 0 2 2 138.0 0 9 9 139.0 0 3 3 140.0 0 5 5 141.0 0 5 5 142.0 0 3 3 143.0 0 7 7 144.0 0 3 3 145.0 0 4 4 147.0 0 3 3 148.0 0 2 2 149.0 0 2 2 150.0 0 7 7 151.0 0 6 6 152.0 0 4 4 153.0 0 4 4 154.0 0 1 1 155.0 0 3 3 156.0 0 3 3 157.0 0 4 4 158.0 0 3 3 159.0 0 4 4 160.0 0 6 6 161.0 0 2 2 162.0 0 6 6 163.0 0 7 7 164.0 0 3 3 165.0 0 3 3 166.0 0 2 2 167.0 0 4 4 168.0 0 3 3 169.0 0 3 3 170.0 0 3 3 171.0 0 3 3 172.0 0 2 2 173.0 0 2 2 174.0 0 1 1 175.0 0 2 2 176.0 0 2 2 177.0 0 1 1 178.0 0 2 2 179.0 0 3 3 180.0 0 2 2 181.0 0 4 4 182.0 0 2 2 183.0 0 1 1 184.0 0 5 5 185.0 0 1 1 186.0 0 4 4 187.0 0 3 3 188.0 0 4 4 189.0 0 4 4 190.0 0 2 2 191.0 0 3 3 192.0 0 3 3 194.0 0 7 7 195.0 0 1 1 196.0 0 5 5 197.0 0 4 4 199.0 0 1 1 200.0 0 3 3 201.0 0 2 2 202.0 0 3 3 203.0 0 2 2 204.0 0 4 4 205.0 0 7 7 206.0 0 3 3 207.0 0 2 2 208.0 0 1 1 209.0 0 3 3 210.0 0 2 2 211.0 0 3 3 212.0 0 1 1 213.0 0 2 2 214.0 0 2 2 216.0 0 2 2 217.0 0 3 3 218.0 0 1 1 219.0 0 2 2 220.0 0 1 1 221.0 0 2 2 222.0 0 2 2 223.0 0 4 4 225.0 0 1 1 226.0 0 1 1 227.0 0 3 3 228.0 0 4 4 229.0 0 2 2 230.0 0 3 3 231.0 0 1 1 232.0 0 1 1 233.0 0 2 2 234.0 0 2 2 235.0 0 4 4 236.0 0 3 3 237.0 0 3 3 238.0 0 3 3 239.0 0 3 3 240.0 0 2 2 241.0 0 3 3 242.0 0 2 2 243.0 0 3 3 244.0 0 3 3 245.0 0 3 3 246.0 0 2 2 247.0 0 1 1 248.0 0 2 2 249.0 0 1 1 251.0 0 2 2 252.0 0 1 1 253.0 0 1 1 254.0 0 2 2 255.0 0 1 1 256.0 0 1 1 257.0 0 1 1 258.0 0 5 5 259.0 0 1 1 260.0 0 1 1 262.0 0 1 1 263.0 0 4 4 265.0 0 4 4 267.0 0 2 2 268.0 0 1 1 270.0 0 1 1 271.0 0 2 2 272.0 0 2 2 274.0 0 2 2 275.0 0 3 3 276.0 0 2 2 277.0 0 1 1 280.0 0 2 2 281.0 0 1 1 283.0 0 2 2 286.0 0 3 3 287.0 0 2 2 288.0 0 1 1 290.0 0 1 1 291.0 0 4 4 292.0 0 2 2 293.0 0 1 1 295.0 0 1 1 296.0 0 2 2 297.0 0 1 1 298.0 0 4 4 299.0 0 1 1 300.0 0 1 1 303.0 0 2 2 304.0 0 1 1 305.0 0 1 1 306.0 0 2 2 307.0 0 2 2 308.0 0 3 3 309.0 0 2 2 311.0 0 2 2 312.0 0 2 2 313.0 0 3 3 315.0 0 2 2 316.0 0 1 1 317.0 0 3 3 318.0 0 4 4 319.0 0 3 3 320.0 0 2 2 322.0 0 1 1 324.0 0 1 1 327.0 0 2 2 332.0 0 1 1 333.0 0 2 2 334.0 0 2 2 335.0 0 2 2 336.0 0 1 1 337.0 0 2 2 338.0 0 1 1 339.0 0 4 4 340.0 0 1 1 342.0 0 3 3 343.0 0 1 1 345.0 0 1 1 346.0 0 2 2 347.0 0 2 2 348.0 0 1 1 349.0 0 1 1 352.0 0 2 2 353.0 0 1 1 354.0 0 2 2 355.0 0 1 1 357.0 0 1 1 359.0 0 2 2 361.0 0 2 2 362.0 0 1 1 365.0 0 2 2 366.0 0 2 2 367.0 0 2 2 370.0 0 3 3 372.0 0 1 1 373.0 0 2 2 374.0 0 1 1 375.0 0 1 1 377.0 0 2 2 379.0 0 1 1 380.0 0 1 1 381.0 0 1 1 384.0 0 1 1 387.0 0 1 1 388.0 0 3 3 391.0 0 2 2 392.0 0 1 1 393.0 0 2 2 394.0 0 1 1 395.0 0 1 1 396.0 0 1 1 397.0 0 1 1 399.0 0 1 1 400.0 0 4 4 401.0 0 2 2 403.0 0 1 1 404.0 0 1 1 405.0 0 3 3 406.0 0 2 2 408.0 0 1 1 410.0 0 1 1 411.0 0 2 2 417.0 0 2 2 418.0 0 1 1 421.0 0 1 1 422.0 0 1 1 423.0 0 2 2 430.0 0 1 1 431.0 0 1 1 432.0 0 1 1 433.0 0 1 1 434.0 0 3 3 435.0 0 1 1 436.0 0 1 1 437.0 0 1 1 439.0 0 2 2 440.0 0 1 1 441.0 0 1 1 443.0 0 1 1 445.0 0 1 1 446.0 0 2 2 448.0 0 1 1 451.0 0 1 1 456.0 0 3 3 457.0 0 1 1 459.0 0 1 1 460.0 0 1 1 461.0 0 1 1 464.0 0 2 2 465.0 0 2 2 467.0 0 1 1 468.0 0 1 1 469.0 0 1 1 470.0 0 1 1 472.0 0 1 1 473.0 0 1 1 474.0 0 1 1 475.0 0 1 1 479.0 0 2 2 481.0 0 1 1 482.0 0 1 1 483.0 0 2 2 485.0 0 1 1 486.0 0 1 1 487.0 0 1 1 490.0 0 1 1 492.0 0 2 2 494.0 0 1 1 495.0 0 1 1 498.0 0 1 1 499.0 0 1 1 500.0 0 1 1 501.0 0 1 1 502.0 0 1 1 503.0 0 1 1 505.0 0 1 1 509.0 0 2 2 512.0 0 1 1 513.0 0 1 1 515.0 0 2 2 517.0 0 1 1 518.0 0 1 1 519.0 0 1 1 520.0 0 1 1 523.0 0 1 1 524.0 0 3 3 527.0 0 1 1 530.0 0 1 1 533.0 0 1 1 534.0 0 1 1 536.0 0 2 2 540.0 0 1 1 541.0 0 1 1 542.0 0 1 1 543.0 0 2 2 545.0 0 1 1 549.0 0 2 2 551.0 0 1 1 552.0 0 1 1 554.0 0 3 3 556.0 0 1 1 557.0 0 1 1 559.0 0 1 1 563.0 0 1 1 566.0 0 1 1 568.0 0 1 1 569.0 0 3 3 572.0 0 1 1 575.0 0 1 1 577.0 0 2 2 578.0 0 1 1 580.0 0 1 1 583.0 0 1 1 585.0 0 1 1 586.0 0 3 3 587.0 0 1 1 588.0 0 1 1 590.0 0 1 1 594.0 0 2 2 597.0 0 2 2 598.0 0 1 1 601.0 0 2 2 606.0 0 1 1 616.0 0 1 1 617.0 0 1 1 618.0 0 1 1 620.0 0 2 2 624.0 0 1 1 626.0 0 1 1 627.0 0 1 1 628.0 0 1 1 630.0 0 2 2 633.0 0 2 2 634.0 0 2 2 636.0 0 1 1 639.0 0 2 2 641.0 0 1 1 646.0 0 1 1 658.0 0 1 1 659.0 0 1 1 662.0 0 1 1 663.0 0 1 1 665.0 0 1 1 673.0 0 2 2 675.0 0 1 1 677.0 0 1 1 680.0 0 1 1 681.0 0 1 1 682.0 0 1 1 683.0 0 1 1 689.0 0 1 1 690.0 0 1 1 692.0 0 1 1 694.0 0 1 1 695.0 0 1 1 697.0 0 1 1 700.0 0 1 1 701.0 0 2 2 708.0 0 1 1 713.0 0 1 1 714.0 0 2 2 716.0 0 1 1 717.0 0 1 1 721.0 0 1 1 728.0 0 1 1 731.0 0 1 1 747.0 0 1 1 757.0 0 1 1 758.0 0 1 1 760.0 0 1 1 764.0 0 1 1 775.0 0 2 2 781.0 0 1 1 786.0 0 1 1 788.0 0 1 1 790.0 0 1 1 792.0 0 1 1 795.0 0 1 1 796.0 0 1 1 801.0 0 1 1 814.0 0 1 1 827.0 0 1 1 828.0 0 2 2 831.0 0 1 1 834.0 0 1 1 840.0 0 1 1 841.0 0 1 1 846.0 0 1 1 848.0 0 1 1 852.0 0 2 2 872.0 0 1 1 875.0 0 1 1 878.0 0 1 1 881.0 0 1 1 893.0 0 1 1 895.0 0 1 1 897.0 0 1 1 905.0 0 1 1 906.0 0 1 1 909.0 0 1 1 915.0 0 1 1 926.0 0 1 1 943.0 0 1 1 944.0 0 1 1 963.0 0 1 1 967.0 0 1 1 968.0 0 1 1 969.0 0 2 2 981.0 0 1 1 987.0 0 2 2 1001.0 0 1 1 1005.0 0 1 1 1015.0 0 1 1 1022.0 0 1 1 1031.0 0 1 1 1037.0 0 1 1 1048.0 0 1 1 1049.0 0 1 1 1058.0 0 1 1 1112.0 0 1 1 1124.0 0 1 1 1138.0 0 1 1 1151.0 0 1 1 1155.0 0 1 1 1165.0 0 1 1 1167.0 0 1 1 1184.0 0 1 1 1232.0 0 1 1 1241.0 0 1 1 1249.0 0 2 2 1255.0 0 1 1 1270.0 0 1 1 1288.0 0 1 1 1338.0 0 1 1 1339.0 0 1 1 1349.0 0 1 1 1354.0 0 1 1 1369.0 0 1 1 1398.0 0 1 1 1410.0 0 1 1 1421.0 0 1 1 1422.0 0 1 1 1463.0 0 1 1 1469.0 0 1 1 1496.0 0 1 1 1513.0 0 1 1 1517.0 0 1 1 1578.0 0 1 1 1629.0 0 1 1 1744.0 0 1 1 1749.0 0 1 1 1756.0 0 1 1 1780.0 0 1 1 1825.0 0 1 1 1840.0 0 1 1 1856.0 0 1 1 1885.0 0 1 1 1934.0 0 1 1 1963.0 0 1 1 2048.0 0 1 1 2122.0 0 1 1 2278.0 0 1 1 2384.0 0 1 1 2506.0 0 1 1 2629.0 0 1 1 2663.0 0 1 1 3267.0 0 1 1 3366.0 0 1 1