## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Esp_1_2012-11-20/US_Cal_Esp_1_2012-11-20.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Esp_1_2012-11-20/US_Cal_Esp_1_2012-11-20.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/US_Cal_Esp_1_2012-11-20/US_Cal_Esp_1_2012-11-20.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 17:13:22 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 965443 26760155 0 0 265707 1652173 0 0.065523 207701385 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 23646641 23646641 2.0 1.879115 945836 945836 3.0 2.651958 35271 35271 4.0 3.331376 3490 3490 5.0 3.928663 1502 1502 6.0 4.453746 1035 1035 7.0 4.915355 690 690 8.0 5.321163 505 505 9.0 5.677914 391 391 10.0 5.991539 300 300 11.0 6.267252 280 280 12.0 6.509635 191 191 13.0 6.722718 179 179 14.0 6.910042 151 151 15.0 7.074722 148 148 16.0 7.219494 126 126 17.0 7.346765 120 120 18.0 7.458652 112 112 19.0 7.557013 98 98 20.0 7.643483 88 88 21.0 7.719501 59 59 22.0 7.786329 69 69 23.0 7.845078 73 73 24.0 7.896726 63 63 25.0 7.94213 57 57 26.0 7.982046 73 73 27.0 8.017136 55 55 28.0 8.047985 46 46 29.0 8.075104 51 51 30.0 8.098945 57 57 31.0 8.119904 61 61 32.0 8.13833 40 40 33.0 8.154528 29 29 34.0 8.168768 51 51 35.0 8.181286 32 32 36.0 8.192291 37 37 37.0 8.201966 33 33 38.0 8.210471 35 35 39.0 8.217949 32 32 40.0 8.224522 25 25 41.0 8.230301 17 17 42.0 8.235381 22 22 43.0 8.239847 34 34 44.0 8.243773 18 18 45.0 8.247224 25 25 46.0 8.250259 20 20 47.0 8.252926 29 29 48.0 8.255271 23 23 49.0 8.257333 21 21 50.0 8.259145 20 20 51.0 8.260738 24 24 52.0 8.262139 22 22 53.0 8.26337 13 13 54.0 8.264453 12 12 55.0 8.265404 19 19 56.0 8.266241 16 16 57.0 8.266976 18 18 58.0 8.267623 13 13 59.0 8.268191 14 14 60.0 8.268691 18 18 61.0 8.26913 14 14 62.0 8.269517 14 14 63.0 8.269856 8 8 64.0 8.270154 12 12 65.0 8.270417 23 23 66.0 8.270647 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