FastQCFastQC Report
Fri 20 Jan 2023
US_Cal_Esp_1_2012-08-22.trimmed2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUS_Cal_Esp_1_2012-08-22.trimmed2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53028072
Sequences flagged as poor quality0
Sequence length75-92
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1062660.20039574510647867No Hit
CTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATT900480.1698119441340428No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA834690.15740530789050752No Hit
ATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAAC709950.13388191824134205No Hit
AGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACAT642770.12121315668425584No Hit
CATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAA611110.11524273407488773No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCT605700.11422251972502413No Hit
ATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTT557130.10506322009972378No Hit
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG546020.1029681033849392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)