## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/UA_Lvi_Dro_1_2014-08-24/UA_Lvi_Dro_1_2014-08-24.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/UA_Lvi_Dro_1_2014-08-24/UA_Lvi_Dro_1_2014-08-24.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/UA_Lvi_Dro_1_2014-08-24/UA_Lvi_Dro_1_2014-08-24.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 16:55:15 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 15914010 15855999 0 0 3123843 542754 0 0.088383 226292289 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 14673289 14673289 2.0 1.93233 330136 330136 3.0 2.801569 6807 6807 4.0 3.611986 713 713 5.0 4.367562 453 453 6.0 5.072008 276 276 7.0 5.728784 205 205 8.0 6.341116 168 168 9.0 6.912012 151 151 10.0 7.444275 133 133 11.0 7.940519 129 129 12.0 8.403182 101 101 13.0 8.834537 78 78 14.0 9.236702 78 78 15.0 9.611653 87 87 16.0 9.96123 59 59 17.0 10.287152 73 73 18.0 10.591018 71 71 19.0 10.874322 42 42 20.0 11.138454 59 59 21.0 11.384713 41 41 22.0 11.614307 51 51 23.0 11.828365 41 41 24.0 12.027937 33 33 25.0 12.214004 29 29 26.0 12.38748 32 32 27.0 12.549216 42 42 28.0 12.700008 35 35 29.0 12.840596 27 27 30.0 12.971671 22 22 31.0 13.093875 22 22 32.0 13.20781 20 20 33.0 13.314035 29 29 34.0 13.413071 16 16 35.0 13.505406 20 20 36.0 13.591492 32 32 37.0 13.671753 21 21 38.0 13.746583 19 19 39.0 13.816349 14 14 40.0 13.881394 11 11 41.0 13.942037 17 17 42.0 13.998577 24 24 43.0 14.05129 18 18 44.0 14.100437 16 16 45.0 14.146257 12 12 46.0 14.188977 20 20 47.0 14.228806 14 14 48.0 14.26594 15 15 49.0 14.300561 13 13 50.0 14.332839 10 10 51.0 14.362933 11 11 52.0 14.39099 8 8 53.0 14.417149 15 15 54.0 14.441538 8 8 55.0 14.464276 9 9 56.0 14.485475 12 12 57.0 14.50524 10 10 58.0 14.523668 10 10 59.0 14.540848 5 5 60.0 14.556866 7 7 61.0 14.5718 8 8 62.0 14.585723 10 10 63.0 14.598705 7 7 64.0 14.610807 11 11 65.0 14.622091 1 1 66.0 14.632611 7 7 67.0 14.642419 12 12 68.0 14.651564 8 8 69.0 14.66009 6 6 70.0 14.668038 5 5 71.0 14.675449 9 9 72.0 14.682359 10 10 73.0 14.6888 3 3 74.0 14.694806 13 13 75.0 14.700406 6 6 76.0 14.705626 5 5 77.0 14.710494 8 8 78.0 14.715032 7 7 79.0 14.719262 6 6 80.0 14.723207 6 6 81.0 14.726885 3 3 82.0 14.730313 8 8 83.0 14.73351 8 8 84.0 14.73649 9 9 85.0 14.739269 5 5 86.0 14.74186 3 3 87.0 14.744275 5 5 88.0 14.746527 8 8 89.0 14.748627 9 9 90.0 14.750584 13 13 91.0 14.752409 5 5 92.0 14.754111 4 4 93.0 14.755697 8 8 94.0 14.757176 7 7 95.0 14.758555 8 8 96.0 14.75984 4 4 97.0 14.761039 5 5 98.0 14.762157 6 6 99.0 14.763198 6 6 100.0 14.76417 5 5 101.0 0 4 4 102.0 0 5 5 103.0 0 4 4 104.0 0 5 5 105.0 0 3 3 106.0 0 5 5 107.0 0 1 1 108.0 0 4 4 109.0 0 6 6 110.0 0 3 3 111.0 0 3 3 112.0 0 1 1 113.0 0 1 1 114.0 0 5 5 115.0 0 5 5 116.0 0 3 3 118.0 0 4 4 119.0 0 4 4 121.0 0 4 4 122.0 0 2 2 123.0 0 4 4 124.0 0 2 2 125.0 0 4 4 126.0 0 2 2 127.0 0 3 3 128.0 0 2 2 129.0 0 1 1 130.0 0 3 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