FastQCFastQC Report
Fri 20 Jan 2023
SRR8439169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8439169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60857084
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1951970.32074655433704313Illumina Single End PCR Primer 1 (100% over 50bp)
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC1031690.16952668977698637No Hit
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC835330.13726093087207397No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA674810.11088437954076143No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA638840.10497381044415469No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG622340.10226254021635346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)