## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/UA_Che_Var_1_2014-08-18/UA_Che_Var_1_2014-08-18.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/UA_Che_Var_1_2014-08-18/UA_Che_Var_1_2014-08-18.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/UA_Che_Var_1_2014-08-18/UA_Che_Var_1_2014-08-18.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 08:46:06 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 8908448 19751018 0 0 1955361 763607 0 0.071938 248807223 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 18142454 18142454 2.0 1.923686 402992 402992 3.0 2.776883 8053 8053 4.0 3.564968 1065 1065 5.0 4.292912 581 581 6.0 4.965304 415 415 7.0 5.586383 298 298 8.0 6.160065 245 245 9.0 6.689968 201 201 10.0 7.179431 157 157 11.0 7.631542 131 131 12.0 8.04915 131 131 13.0 8.434889 90 90 14.0 8.791191 100 100 15.0 9.120303 83 83 16.0 9.424298 106 106 17.0 9.705095 79 79 18.0 9.964463 73 73 19.0 10.204037 60 60 20.0 10.425329 61 61 21.0 10.629733 52 52 22.0 10.818538 60 60 23.0 10.992935 40 40 24.0 11.154023 53 53 25.0 11.302818 41 41 26.0 11.440257 41 41 27.0 11.567209 27 27 28.0 11.684472 44 44 29.0 11.792786 38 38 30.0 11.892834 34 34 31.0 11.985247 37 37 32.0 12.070608 46 46 33.0 12.149455 20 20 34.0 12.222285 22 22 35.0 12.289556 34 34 36.0 12.351694 29 29 37.0 12.40909 26 26 38.0 12.462106 21 21 39.0 12.511076 29 29 40.0 12.556309 20 20 41.0 12.59809 31 31 42.0 12.636683 24 24 43.0 12.67233 20 20 44.0 12.705257 10 10 45.0 12.735671 15 15 46.0 12.763765 20 20 47.0 12.789714 16 16 48.0 12.813683 11 11 49.0 12.835823 12 12 50.0 12.856273 20 20 51.0 12.875163 16 16 52.0 12.892611 11 11 53.0 12.908727 12 12 54.0 12.923614 19 19 55.0 12.937365 16 16 56.0 12.950066 13 13 57.0 12.961798 15 15 58.0 12.972635 7 7 59.0 12.982644 16 16 60.0 12.99189 15 15 61.0 13.00043 12 12 62.0 13.008319 6 6 63.0 13.015605 5 5 64.0 13.022336 7 7 65.0 13.028552 15 15 66.0 13.034295 11 11 67.0 13.039599 11 11 68.0 13.044498 8 8 69.0 13.049024 16 16 70.0 13.053204 8 8 71.0 13.057065 11 11 72.0 13.060632 8 8 73.0 13.063926 8 8 74.0 13.066969 10 10 75.0 13.069779 8 8 76.0 13.072376 10 10 77.0 13.074774 5 5 78.0 13.076989 5 5 79.0 13.079035 8 8 80.0 13.080925 7 7 81.0 13.08267 7 7 82.0 13.084283 7 7 83.0 13.085772 11 11 84.0 13.087148 12 12 85.0 13.088419 12 12 86.0 13.089592 5 5 87.0 13.090676 11 11 88.0 13.091678 8 8 89.0 13.092603 11 11 90.0 13.093457 9 9 91.0 13.094247 5 5 92.0 13.094976 5 5 93.0 13.095649 7 7 94.0 13.096271 7 7 95.0 13.096845 6 6 96.0 13.097376 4 4 97.0 13.097866 5 5 98.0 13.098319 5 5 99.0 13.098737 10 10 100.0 13.099124 11 11 101.0 0 8 8 102.0 0 9 9 103.0 0 5 5 104.0 0 5 5 105.0 0 6 6 106.0 0 5 5 107.0 0 4 4 108.0 0 7 7 109.0 0 5 5 110.0 0 1 1 111.0 0 5 5 112.0 0 8 8 113.0 0 8 8 114.0 0 4 4 115.0 0 4 4 116.0 0 4 4 117.0 0 1 1 118.0 0 9 9 119.0 0 4 4 120.0 0 2 2 121.0 0 4 4 122.0 0 3 3 123.0 0 6 6 124.0 0 7 7 125.0 0 6 6 126.0 0 3 3 127.0 0 4 4 128.0 0 6 6 129.0 0 4 4 131.0 0 3 3 132.0 0 6 6 133.0 0 3 3 134.0 0 5 5 135.0 0 3 3 136.0 0 5 5 137.0 0 6 6 138.0 0 3 3 139.0 0 3 3 140.0 0 4 4 141.0 0 4 4 142.0 0 9 9 143.0 0 2 2 144.0 0 4 4 145.0 0 4 4 146.0 0 5 5 147.0 0 2 2 148.0 0 3 3 149.0 0 1 1 150.0 0 3 3 151.0 0 7 7 152.0 0 2 2 153.0 0 1 1 154.0 0 2 2 155.0 0 4 4 157.0 0 4 4 158.0 0 2 2 160.0 0 6 6 161.0 0 5 5 162.0 0 2 2 163.0 0 6 6 165.0 0 2 2 166.0 0 6 6 167.0 0 2 2 168.0 0 3 3 169.0 0 3 3 170.0 0 4 4 171.0 0 4 4 172.0 0 2 2 173.0 0 3 3 174.0 0 1 1 175.0 0 2 2 176.0 0 1 1 177.0 0 2 2 178.0 0 2 2 179.0 0 1 1 180.0 0 1 1 182.0 0 2 2 183.0 0 4 4 184.0 0 4 4 185.0 0 2 2 186.0 0 3 3 187.0 0 1 1 189.0 0 3 3 190.0 0 3 3 191.0 0 3 3 192.0 0 1 1 193.0 0 1 1 194.0 0 2 2 195.0 0 4 4 196.0 0 4 4 197.0 0 2 2 198.0 0 4 4 199.0 0 1 1 200.0 0 1 1 201.0 0 3 3 202.0 0 3 3 203.0 0 1 1 204.0 0 2 2 205.0 0 3 3 206.0 0 2 2 207.0 0 3 3 208.0 0 4 4 211.0 0 1 1 212.0 0 1 1 213.0 0 1 1 215.0 0 3 3 216.0 0 1 1 218.0 0 2 2 219.0 0 3 3 220.0 0 1 1 223.0 0 3 3 224.0 0 3 3 226.0 0 1 1 227.0 0 2 2 228.0 0 2 2 230.0 0 1 1 231.0 0 3 3 232.0 0 2 2 233.0 0 2 2 234.0 0 1 1 235.0 0 2 2 239.0 0 2 2 242.0 0 1 1 245.0 0 2 2 246.0 0 1 1 248.0 0 3 3 249.0 0 1 1 250.0 0 1 1 252.0 0 1 1 253.0 0 1 1 254.0 0 1 1 255.0 0 1 1 256.0 0 3 3 257.0 0 1 1 259.0 0 2 2 260.0 0 1 1 262.0 0 1 1 263.0 0 1 1 264.0 0 1 1 265.0 0 1 1 266.0 0 1 1 267.0 0 1 1 268.0 0 2 2 269.0 0 3 3 270.0 0 1 1 271.0 0 1 1 272.0 0 1 1 275.0 0 2 2 276.0 0 1 1 277.0 0 1 1 278.0 0 2 2 279.0 0 1 1 280.0 0 2 2 281.0 0 1 1 282.0 0 1 1 284.0 0 3 3 285.0 0 4 4 286.0 0 1 1 288.0 0 2 2 289.0 0 4 4 290.0 0 2 2 291.0 0 2 2 292.0 0 3 3 293.0 0 1 1 295.0 0 3 3 296.0 0 3 3 298.0 0 2 2 299.0 0 1 1 300.0 0 1 1 301.0 0 2 2 302.0 0 1 1 305.0 0 3 3 309.0 0 2 2 311.0 0 2 2 312.0 0 3 3 314.0 0 1 1 315.0 0 2 2 317.0 0 1 1 319.0 0 1 1 320.0 0 1 1 321.0 0 2 2 323.0 0 1 1 326.0 0 1 1 328.0 0 3 3 330.0 0 2 2 334.0 0 1 1 336.0 0 2 2 337.0 0 1 1 338.0 0 1 1 339.0 0 1 1 345.0 0 2 2 346.0 0 1 1 347.0 0 1 1 348.0 0 1 1 349.0 0 1 1 350.0 0 3 3 352.0 0 1 1 357.0 0 1 1 358.0 0 1 1 360.0 0 2 2 363.0 0 2 2 366.0 0 1 1 368.0 0 1 1 369.0 0 1 1 372.0 0 1 1 375.0 0 1 1 376.0 0 1 1 378.0 0 1 1 382.0 0 1 1 385.0 0 1 1 386.0 0 1 1 388.0 0 1 1 390.0 0 1 1 392.0 0 1 1 398.0 0 1 1 400.0 0 1 1 405.0 0 1 1 407.0 0 5 5 409.0 0 1 1 411.0 0 2 2 412.0 0 1 1 413.0 0 1 1 414.0 0 1 1 419.0 0 2 2 421.0 0 1 1 422.0 0 1 1 426.0 0 3 3 427.0 0 2 2 432.0 0 1 1 434.0 0 1 1 435.0 0 1 1 436.0 0 1 1 439.0 0 1 1 442.0 0 1 1 445.0 0 1 1 451.0 0 2 2 454.0 0 1 1 457.0 0 1 1 460.0 0 2 2 461.0 0 1 1 464.0 0 2 2 465.0 0 1 1 468.0 0 1 1 469.0 0 1 1 470.0 0 3 3 471.0 0 1 1 475.0 0 1 1 476.0 0 1 1 481.0 0 1 1 482.0 0 2 2 483.0 0 1 1 485.0 0 1 1 492.0 0 2 2 493.0 0 3 3 494.0 0 1 1 495.0 0 1 1 499.0 0 1 1 500.0 0 1 1 508.0 0 1 1 510.0 0 2 2 511.0 0 1 1 518.0 0 1 1 523.0 0 1 1 524.0 0 1 1 527.0 0 1 1 528.0 0 2 2 531.0 0 1 1 538.0 0 1 1 540.0 0 3 3 541.0 0 1 1 549.0 0 3 3 550.0 0 1 1 553.0 0 1 1 562.0 0 1 1 563.0 0 1 1 564.0 0 1 1 566.0 0 1 1 575.0 0 1 1 579.0 0 2 2 580.0 0 1 1 583.0 0 1 1 595.0 0 1 1 598.0 0 3 3 601.0 0 1 1 606.0 0 2 2 617.0 0 1 1 618.0 0 1 1 622.0 0 2 2 627.0 0 1 1 629.0 0 1 1 631.0 0 1 1 632.0 0 1 1 633.0 0 1 1 635.0 0 1 1 646.0 0 1 1 647.0 0 1 1 652.0 0 1 1 656.0 0 1 1 657.0 0 1 1 660.0 0 1 1 661.0 0 2 2 669.0 0 1 1 670.0 0 1 1 677.0 0 1 1 679.0 0 2 2 682.0 0 1 1 683.0 0 1 1 688.0 0 1 1 692.0 0 1 1 695.0 0 1 1 696.0 0 1 1 699.0 0 1 1 705.0 0 1 1 708.0 0 1 1 713.0 0 1 1 719.0 0 1 1 723.0 0 1 1 728.0 0 2 2 729.0 0 1 1 738.0 0 1 1 740.0 0 1 1 744.0 0 1 1 746.0 0 1 1 772.0 0 1 1 780.0 0 1 1 781.0 0 1 1 787.0 0 1 1 790.0 0 1 1 804.0 0 1 1 815.0 0 1 1 818.0 0 1 1 820.0 0 2 2 829.0 0 1 1 839.0 0 1 1 840.0 0 1 1 845.0 0 1 1 851.0 0 2 2 855.0 0 1 1 875.0 0 1 1 910.0 0 2 2 928.0 0 1 1 935.0 0 1 1 940.0 0 1 1 941.0 0 1 1 951.0 0 1 1 953.0 0 1 1 959.0 0 1 1 962.0 0 1 1 964.0 0 1 1 967.0 0 1 1 977.0 0 1 1 988.0 0 1 1 995.0 0 1 1 998.0 0 1 1 1012.0 0 1 1 1017.0 0 1 1 1021.0 0 1 1 1041.0 0 1 1 1058.0 0 1 1 1062.0 0 1 1 1069.0 0 1 1 1072.0 0 2 2 1114.0 0 1 1 1117.0 0 1 1 1139.0 0 1 1 1169.0 0 1 1 1179.0 0 1 1 1187.0 0 1 1 1193.0 0 1 1 1207.0 0 1 1 1232.0 0 1 1 1247.0 0 1 1 1248.0 0 1 1 1256.0 0 1 1 1265.0 0 1 1 1272.0 0 1 1 1290.0 0 1 1 1395.0 0 1 1 1446.0 0 1 1 1471.0 0 1 1 1481.0 0 1 1 1486.0 0 1 1 1603.0 0 1 1 1619.0 0 1 1 1626.0 0 1 1 1633.0 0 1 1 1857.0 0 1 1 2104.0 0 1 1