## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/FR_Dro_Got_1_2014-07-08/FR_Dro_Got_1_2014-07-08.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/FR_Dro_Got_1_2014-07-08/FR_Dro_Got_1_2014-07-08.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/FR_Dro_Got_1_2014-07-08/FR_Dro_Got_1_2014-07-08.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 10:43:33 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 3600100 47052887 0 0 1405372 1778464 0 0.050788 606654832 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 42783983 42783983 2.0 1.92537 1467592 1467592 3.0 2.781681 45781 45781 4.0 3.574085 2190 2190 5.0 4.307352 582 582 6.0 4.985896 387 387 7.0 5.613801 281 281 8.0 6.194845 202 202 9.0 6.732526 207 207 10.0 7.23008 135 135 11.0 7.690502 100 100 12.0 8.116563 99 99 13.0 8.510827 108 108 14.0 8.875667 100 100 15.0 9.213279 91 91 16.0 9.525695 82 82 17.0 9.814796 81 81 18.0 10.082322 50 50 19.0 10.329882 51 51 20.0 10.558967 52 52 21.0 10.770955 48 48 22.0 10.967123 49 49 23.0 11.14865 55 55 24.0 11.316631 42 42 25.0 11.472075 50 50 26.0 11.615918 33 33 27.0 11.749027 40 40 28.0 11.872201 36 36 29.0 11.986183 36 36 30.0 12.091659 23 23 31.0 12.189263 26 26 32.0 12.279583 32 32 33.0 12.363162 29 29 34.0 12.440504 22 22 35.0 12.512074 26 26 36.0 12.578302 16 16 37.0 12.639588 22 22 38.0 12.696301 25 25 39.0 12.74878 23 23 40.0 12.797344 23 23 41.0 12.842283 19 19 42.0 12.883868 17 17 43.0 12.92235 24 24 44.0 12.95796 19 19 45.0 12.990912 19 19 46.0 13.021405 13 13 47.0 13.049622 12 12 48.0 13.075734 9 9 49.0 13.099897 11 11 50.0 13.122257 20 20 51.0 13.142947 14 14 52.0 13.162094 13 13 53.0 13.179812 11 11 54.0 13.196208 15 15 55.0 13.21138 13 13 56.0 13.225419 8 8 57.0 13.238411 19 19 58.0 13.250433 9 9 59.0 13.261559 16 16 60.0 13.271853 14 14 61.0 13.28138 11 11 62.0 13.290196 15 15 63.0 13.298353 12 12 64.0 13.305902 11 11 65.0 13.312888 8 8 66.0 13.319352 12 12 67.0 13.325334 8 8 68.0 13.330869 11 11 69.0 13.335991 5 5 70.0 13.340731 13 13 71.0 13.345117 7 7 72.0 13.349176 14 14 73.0 13.352932 6 6 74.0 13.356408 4 4 75.0 13.359624 12 12 76.0 13.3626 7 7 77.0 13.365355 5 5 78.0 13.367903 7 7 79.0 13.370262 9 9 80.0 13.372444 11 11 81.0 13.374463 9 9 82.0 13.376332 8 8 83.0 13.378062 7 7 84.0 13.379662 7 7 85.0 13.381143 5 5 86.0 13.382513 5 5 87.0 13.383781 9 9 88.0 13.384955 8 8 89.0 13.38604 5 5 90.0 13.387045 7 7 91.0 13.387975 3 3 92.0 13.388835 4 4 93.0 13.389632 8 8 94.0 13.390368 4 4 95.0 13.39105 5 5 96.0 13.391681 1 1 97.0 13.392265 3 3 98.0 13.392805 5 5 99.0 13.393305 5 5 100.0 13.393768 5 5 101.0 0 6 6 102.0 0 5 5 103.0 0 5 5 104.0 0 9 9 105.0 0 1 1 106.0 0 5 5 107.0 0 4 4 108.0 0 6 6 109.0 0 3 3 110.0 0 5 5 111.0 0 5 5 112.0 0 1 1 113.0 0 4 4 114.0 0 3 3 115.0 0 4 4 116.0 0 4 4 117.0 0 3 3 118.0 0 4 4 119.0 0 3 3 120.0 0 6 6 121.0 0 4 4 122.0 0 4 4 123.0 0 4 4 124.0 0 3 3 125.0 0 3 3 126.0 0 4 4 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222.0 0 1 1 224.0 0 2 2 226.0 0 2 2 227.0 0 1 1 228.0 0 2 2 229.0 0 3 3 231.0 0 3 3 232.0 0 4 4 234.0 0 2 2 235.0 0 1 1 236.0 0 1 1 237.0 0 2 2 238.0 0 1 1 239.0 0 1 1 240.0 0 2 2 244.0 0 2 2 247.0 0 2 2 248.0 0 2 2 249.0 0 1 1 250.0 0 1 1 251.0 0 2 2 252.0 0 2 2 253.0 0 1 1 254.0 0 3 3 257.0 0 1 1 258.0 0 1 1 259.0 0 4 4 260.0 0 1 1 262.0 0 1 1 263.0 0 2 2 264.0 0 4 4 267.0 0 1 1 273.0 0 1 1 275.0 0 1 1 276.0 0 1 1 277.0 0 1 1 279.0 0 1 1 280.0 0 1 1 281.0 0 2 2 282.0 0 1 1 285.0 0 3 3 287.0 0 1 1 288.0 0 1 1 289.0 0 1 1 291.0 0 1 1 299.0 0 1 1 300.0 0 1 1 301.0 0 1 1 302.0 0 2 2 304.0 0 1 1 305.0 0 1 1 306.0 0 1 1 307.0 0 2 2 308.0 0 2 2 313.0 0 1 1 314.0 0 1 1 316.0 0 2 2 317.0 0 1 1 318.0 0 1 1 321.0 0 1 1 322.0 0 1 1 326.0 0 1 1 327.0 0 1 1 328.0 0 1 1 331.0 0 1 1 333.0 0 1 1 334.0 0 1 1 338.0 0 1 1 340.0 0 3 3 341.0 0 2 2 344.0 0 1 1 345.0 0 1 1 346.0 0 1 1 347.0 0 2 2 349.0 0 1 1 352.0 0 1 1 353.0 0 1 1 354.0 0 3 3 355.0 0 1 1 363.0 0 1 1 365.0 0 1 1 369.0 0 1 1 370.0 0 1 1 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