FastQCFastQC Report
Fri 20 Jan 2023
SRR8494460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8494460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52490895
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1800290.3429718620724604Illumina Single End PCR Primer 1 (100% over 50bp)
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC630880.12018846316108726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)