FastQCFastQC Report
Fri 20 Jan 2023
SRR3590552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81116376
Sequences flagged as poor quality0
Sequence length92
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG5644170.6958114105097595Illumina Paired End PCR Primer 2 (100% over 43bp)
TGCACTGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG2889120.35616975787971594Illumina Paired End PCR Primer 2 (100% over 43bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT2751250.3391731898870827Illumina Paired End PCR Primer 2 (100% over 50bp)
GTCTCGAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG2225570.2743675333819154Illumina Paired End PCR Primer 2 (100% over 43bp)
GCTATCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1664020.20513983514253645Illumina Paired End PCR Primer 2 (100% over 43bp)
AGCTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTAT1594180.19652998304559363Illumina Paired End PCR Primer 2 (100% over 46bp)
CGTAGCTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1382590.17044523783952084Illumina Paired End PCR Primer 2 (100% over 43bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCG1153110.14215501935145625Illumina Paired End PCR Primer 2 (100% over 50bp)
ATGACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA1032780.12732077675659473Illumina Paired End PCR Primer 2 (100% over 45bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)