FastQCFastQC Report
Fri 20 Jan 2023
SRR3590553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39681985
Sequences flagged as poor quality0
Sequence length92
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT6303281.5884487633368138Illumina Single End PCR Primer 1 (100% over 43bp)
ACGCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT4792541.2077369617472513Illumina Single End PCR Primer 1 (100% over 43bp)
GACGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT4306291.0852002489290795Illumina Single End PCR Primer 1 (100% over 43bp)
TCAGCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG3417270.8611640773514732Illumina Single End PCR Primer 1 (100% over 42bp)
CGACGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT3002880.7567363376605278Illumina Single End PCR Primer 1 (100% over 43bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2757470.6948921532025175No Hit
AGCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1712170.43147287112779265Illumina Single End PCR Primer 1 (100% over 43bp)
GTCACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT910890.22954748861479585Illumina Single End PCR Primer 1 (100% over 43bp)
TACGCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG758040.19102875019987028Illumina Single End PCR Primer 1 (100% over 42bp)
TGACGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG752720.18968809146014243Illumina Single End PCR Primer 1 (100% over 42bp)
AGACTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG733590.18486726407461723Illumina Single End PCR Primer 1 (100% over 45bp)
AGTCACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG607200.15301653886518027Illumina Single End PCR Primer 1 (100% over 42bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC531150.13385167097865694Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)