FastQCFastQC Report
Fri 20 Jan 2023
SRR3590553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39681985
Sequences flagged as poor quality0
Sequence length92
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGTAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG5812371.4647377141037679Illumina Paired End PCR Primer 2 (100% over 43bp)
GACGTACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG4931751.2428183721152053Illumina Paired End PCR Primer 2 (100% over 43bp)
AGCGATCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG3950380.9955096752342404Illumina Paired End PCR Primer 2 (100% over 43bp)
CAGCGATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG2633800.6637268775742947Illumina Paired End PCR Primer 2 (100% over 43bp)
AGTCACGTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTC2459090.6196993421574047Illumina Paired End PCR Primer 2 (100% over 42bp)
ACGCAGTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1557760.3925610072177589Illumina Paired End PCR Primer 2 (100% over 43bp)
TCAGCATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1459940.3679100226462965Illumina Paired End PCR Primer 2 (100% over 43bp)
TGACGTACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTC1107240.27902838025869925Illumina Paired End PCR Primer 2 (100% over 42bp)
TGACGTAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1045400.2634444824269754Illumina Paired End PCR Primer 2 (100% over 43bp)
AGACTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA843950.21267837281829524Illumina Paired End PCR Primer 2 (100% over 45bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCG541870.1365531487399131Illumina Paired End PCR Primer 2 (100% over 50bp)
CAGCATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT464960.11717155782403525Illumina Paired End PCR Primer 2 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)