## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/project/berglandlab/DEST/dest_mapped/DESTv1/DE_Bay_Mun_1_2014-09-03/DE_Bay_Mun_1_2014-09-03.sorted_merged.bam] OUTPUT=/project/berglandlab/DEST/dest_mapped/DESTv1/DE_Bay_Mun_1_2014-09-03/DE_Bay_Mun_1_2014-09-03.dedup.bam METRICS_FILE=/project/berglandlab/DEST/dest_mapped/DESTv1/DE_Bay_Mun_1_2014-09-03/DE_Bay_Mun_1_2014-09-03.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Fri Jan 20 08:35:21 UTC 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 8234036 17652434 0 0 1691347 551563 0 0.064183 276562328 ## HISTOGRAM java.lang.Double BIN VALUE all_sets non_optical_sets 1.0 1 16400336 16400336 2.0 1.938166 344276 344276 3.0 2.818322 6653 6653 4.0 3.644055 791 791 5.0 4.41873 485 485 6.0 5.145503 334 334 7.0 5.827338 272 272 8.0 6.467012 207 207 9.0 7.067133 152 152 10.0 7.630146 150 150 11.0 8.158346 117 117 12.0 8.653885 102 102 13.0 9.118783 91 91 14.0 9.554935 84 84 15.0 9.964118 77 77 16.0 10.348 84 84 17.0 10.708145 59 59 18.0 11.046021 65 65 19.0 11.363004 68 68 20.0 11.660388 48 48 21.0 11.939383 42 42 22.0 12.201127 52 52 23.0 12.446686 43 43 24.0 12.677061 50 50 25.0 12.893192 38 38 26.0 13.095958 44 44 27.0 13.286187 37 37 28.0 13.464653 34 34 29.0 13.632083 39 39 30.0 13.789161 27 27 31.0 13.936526 25 25 32.0 14.07478 27 27 33.0 14.204484 25 25 34.0 14.326168 26 26 35.0 14.440328 23 23 36.0 14.547429 21 21 37.0 14.647908 21 21 38.0 14.742174 25 25 39.0 14.830611 25 25 40.0 14.913579 18 18 41.0 14.991417 16 16 42.0 15.064443 15 15 43.0 15.132952 8 8 44.0 15.197226 17 17 45.0 15.257525 14 14 46.0 15.314096 16 16 47.0 15.367169 14 14 48.0 15.41696 16 16 49.0 15.463673 16 16 50.0 15.507497 18 18 51.0 15.548611 15 15 52.0 15.587183 17 17 53.0 15.62337 12 12 54.0 15.657319 12 12 55.0 15.689169 11 11 56.0 15.71905 7 7 57.0 15.747083 13 13 58.0 15.773382 20 20 59.0 15.798056 10 10 60.0 15.821204 10 10 61.0 15.84292 11 11 62.0 15.863294 13 13 63.0 15.882408 8 8 64.0 15.90034 4 4 65.0 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