FastQCFastQC Report
Fri 20 Jan 2023
SRR3590548_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590548_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50635328
Sequences flagged as poor quality0
Sequence length92
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3071630.6066179723374163No Hit
CTGTAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT2204130.4352948992450489Illumina Single End PCR Primer 1 (100% over 43bp)
GTCTCGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1285860.2539452297020768Illumina Single End PCR Primer 1 (100% over 43bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1221970.2413275569183634Illumina Single End PCR Primer 1 (100% over 50bp)
TCGTGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1014470.20034826277811413Illumina Single End PCR Primer 1 (100% over 43bp)
AGTCTCGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG924280.182536587893733Illumina Single End PCR Primer 1 (100% over 42bp)
TGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT878770.17354879186326197Illumina Single End PCR Primer 1 (100% over 46bp)
ATGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG703060.13884772307587304Illumina Single End PCR Primer 1 (100% over 45bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC607670.12000909720580855Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)