FastQCFastQC Report
Fri 20 Jan 2023
SRR3590548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50635328
Sequences flagged as poor quality0
Sequence length92
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG2174730.4294886763644545Illumina Paired End PCR Primer 2 (100% over 43bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT1239500.24478956668356136Illumina Paired End PCR Primer 2 (100% over 50bp)
AGCTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTAT954910.1885857241805563Illumina Paired End PCR Primer 2 (100% over 46bp)
GTCTCGAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG827730.16346887295763146Illumina Paired End PCR Primer 2 (100% over 43bp)
ATGACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA704460.13912420987971086Illumina Paired End PCR Primer 2 (100% over 45bp)
GCTATCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG690460.13635934184133258Illumina Paired End PCR Primer 2 (100% over 43bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCG629200.124261069267686Illumina Paired End PCR Primer 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)