FastQCFastQC Report
Fri 20 Jan 2023
SRR3590549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36851652
Sequences flagged as poor quality0
Sequence length92
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2440900.662358365915319No Hit
GACGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT2288190.6209192467138244Illumina Single End PCR Primer 1 (100% over 43bp)
CAGCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT2238480.6074300278315881Illumina Single End PCR Primer 1 (100% over 43bp)
ACGCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1728310.4689911866094904Illumina Single End PCR Primer 1 (100% over 43bp)
CGACGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1687480.457911629036332Illumina Single End PCR Primer 1 (100% over 43bp)
TCAGCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG1231470.33416955093356465Illumina Single End PCR Primer 1 (100% over 42bp)
AGCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT958800.26017829539907733Illumina Single End PCR Primer 1 (100% over 43bp)
AGACTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG428460.11626615816300447Illumina Single End PCR Primer 1 (100% over 45bp)
GTCACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT416360.11298272327113042Illumina Single End PCR Primer 1 (100% over 43bp)
TGACGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG411990.11179688769447839Illumina Single End PCR Primer 1 (100% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)