FastQCFastQC Report
Fri 20 Jan 2023
SRR3590549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36851652
Sequences flagged as poor quality0
Sequence length92
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGTAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG2528520.6861347762645755Illumina Paired End PCR Primer 2 (100% over 43bp)
GACGTACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG2025470.5496280058218286Illumina Paired End PCR Primer 2 (100% over 43bp)
AGCGATCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1762960.47839375016349334Illumina Paired End PCR Primer 2 (100% over 43bp)
CAGCGATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1317150.3574195262670992Illumina Paired End PCR Primer 2 (100% over 43bp)
AGTCACGTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTC973280.26410756293910514Illumina Paired End PCR Primer 2 (100% over 42bp)
TCAGCATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG609130.16529245418902794Illumina Paired End PCR Primer 2 (100% over 43bp)
TGACGTACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTC473170.12839858576760682Illumina Paired End PCR Primer 2 (100% over 42bp)
AGACTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA447300.12137854769712901Illumina Paired End PCR Primer 2 (100% over 45bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)