## htsjdk.samtools.metrics.StringHeader # MarkDuplicates INPUT=[/scratch/yey2sn/DEST_example/pipeline_output/VA_ch_18_m08d23/VA_ch_18_m08d23.sorted_merged.bam] OUTPUT=/scratch/yey2sn/DEST_example/pipeline_output/VA_ch_18_m08d23/VA_ch_18_m08d23.dedup.bam METRICS_FILE=/scratch/yey2sn/DEST_example/pipeline_output/VA_ch_18_m08d23/VA_ch_18_m08d23.mark_duplicates_report.txt REMOVE_DUPLICATES=true VALIDATION_STRINGENCY=SILENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Mon Feb 15 22:01:44 UTC 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE lib1 53132151 42365782 0 0 28222539 2768516 236690 0.244876 336321561 ## HISTOGRAM java.lang.Double BIN VALUE all_sets optical_sets non_optical_sets 1.0 1.005272 35770344 0 35983246 2.0 1.891563 2313923 233298 2122676 3.0 2.672955 118319 1634 98171 4.0 3.361864 7733 22 6323 5.0 3.969235 1643 2 1569 6.0 4.50472 933 6 928 7.0 4.976827 658 1 652 8.0 5.393056 491 2 487 9.0 5.760022 420 0 418 10.0 6.083555 351 0 353 11.0 6.368795 270 0 271 12.0 6.620276 226 0 221 13.0 6.841991 175 0 176 14.0 7.037465 168 0 169 15.0 7.209804 159 0 156 16.0 7.361745 164 0 168 17.0 7.495703 122 0 118 18.0 7.613805 112 0 116 19.0 7.71793 86 0 86 20.0 7.809731 103 0 98 21.0 7.890666 86 0 86 22.0 7.962022 76 0 76 23.0 8.024933 40 0 42 24.0 8.080398 68 0 70 25.0 8.129298 49 0 48 26.0 8.17241 56 0 53 27.0 8.21042 49 0 50 28.0 8.243931 43 0 41 29.0 8.273476 34 0 38 30.0 8.299524 41 0 40 31.0 8.322489 28 0 29 32.0 8.342736 29 0 29 33.0 8.360586 34 0 32 34.0 8.376324 27 0 28 35.0 8.390199 31 0 29 36.0 8.402432 29 0 30 37.0 8.413217 22 0 22 38.0 8.422726 27 0 26 39.0 8.431109 24 0 24 40.0 8.4385 16 0 14 41.0 8.445016 9 0 12 42.0 8.450761 20 0 18 43.0 8.455826 14 0 18 44.0 8.460291 15 0 14 45.0 8.464228 17 0 14 46.0 8.467699 11 0 14 47.0 8.47076 11 0 9 48.0 8.473458 11 0 10 49.0 8.475836 11 0 10 50.0 8.477933 8 0 8 51.0 8.479782 7 0 9 52.0 8.481412 7 0 8 53.0 8.48285 9 0 11 54.0 8.484117 14 0 11 55.0 8.485234 12 0 11 56.0 8.486219 11 0 13 57.0 8.487087 14 0 14 58.0 8.487853 8 0 5 59.0 8.488527 10 0 12 60.0 8.489123 6 0 8 61.0 8.489647 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