FastQCFastQC Report
Mon 15 Feb 2021
VA_ch_17_m08d31_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameVA_ch_17_m08d31_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114854330
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT60214785.2427087424566405TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTAT2114440.1840975433838672TruSeq Adapter, Index 2 (97% over 37bp)
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1261800.10986089945411723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)